NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 2D 1H-15N HSQC | 1 mM [U-13C; U-15N] DA05R, 25 mM sodium phosphate, 50 mM sodium chloride, 0.02 % sodium azide, 1 mM protein inhibitor cocktail, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
2 | 2D CT 1H-13C HSQC aliphatic | 1 mM [U-13C; U-15N] DA05R, 25 mM sodium phosphate, 50 mM sodium chloride, 0.02 % sodium azide, 1 mM protein inhibitor cocktail, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
3 | 2D CT 1H-13C HSQC aromatic | 1 mM [U-13C; U-15N] DA05R, 25 mM sodium phosphate, 50 mM sodium chloride, 0.02 % sodium azide, 1 mM protein inhibitor cocktail, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
4 | 3D 15N/13C-edited 1H-1H NOESY | 1 mM [U-13C; U-15N] DA05R, 25 mM sodium phosphate, 50 mM sodium chloride, 0.02 % sodium azide, 1 mM protein inhibitor cocktail, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
5 | (4,3)D GFT HCCH-COSY aliphatic | 1 mM [U-13C; U-15N] DA05R, 25 mM sodium phosphate, 50 mM sodium chloride, 0.02 % sodium azide, 1 mM protein inhibitor cocktail, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
6 | (4,3)D GFT HCCH-COSY aromatic | 1 mM [U-13C; U-15N] DA05R, 25 mM sodium phosphate, 50 mM sodium chloride, 0.02 % sodium azide, 1 mM protein inhibitor cocktail, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
7 | 3D HCCH-TOCSY | 1 mM [U-13C; U-15N] DA05R, 25 mM sodium phosphate, 50 mM sodium chloride, 0.02 % sodium azide, 1 mM protein inhibitor cocktail, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
8 | 3D HNCO | 1 mM [U-13C; U-15N] DA05R, 25 mM sodium phosphate, 50 mM sodium chloride, 0.02 % sodium azide, 1 mM protein inhibitor cocktail, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
9 | 3D HN(CA)CO | 1 mM [U-13C; U-15N] DA05R, 25 mM sodium phosphate, 50 mM sodium chloride, 0.02 % sodium azide, 1 mM protein inhibitor cocktail, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
10 | 3D HNCACB | 1 mM [U-13C; U-15N] DA05R, 25 mM sodium phosphate, 50 mM sodium chloride, 0.02 % sodium azide, 1 mM protein inhibitor cocktail, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
11 | 3D CBCA(CO)NH | 1 mM [U-13C; U-15N] DA05R, 25 mM sodium phosphate, 50 mM sodium chloride, 0.02 % sodium azide, 1 mM protein inhibitor cocktail, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
12 | 3D HBHA(CO)NH | 1 mM [U-13C; U-15N] DA05R, 25 mM sodium phosphate, 50 mM sodium chloride, 0.02 % sodium azide, 1 mM protein inhibitor cocktail, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
13 | 2D CT 1H-13C HSQC aliphatic 28 ms | 660 uM [U-5% 13C; U-15N] DA05R, 25 mM sodium phosphate, 50 mM sodium chloride, 0.02 % sodium azide, 1 mM protein inhibitor cocktail, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
14 | 3D 1H-15N TOCSY | 660 uM [U-5% 13C; U-15N] DA05R, 25 mM sodium phosphate, 50 mM sodium chloride, 0.02 % sodium azide, 1 mM protein inhibitor cocktail, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
15 | 3D 1H-15N NOESY | 660 uM [U-5% 13C; U-15N] DA05R, 25 mM sodium phosphate, 50 mM sodium chloride, 0.02 % sodium azide, 1 mM protein inhibitor cocktail, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |