| NMR Experiment |
|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
|---|
| 1 | 2D 1H-15N HSQC | 1 mM [U-100% 13C; U-100% 15N] MvaT, 50 mM sodium phosphate, 50 mM sodium chloride, 1 mM DNA (5'-D(*CP*GP*CP*AP*TP*AP*TP*AP*TP*GP*CP*G)-3') | 90% H2O/10% D2O | 0.1 | 6 | ambient | 298 | |
| 2 | 3D HCCH-TOCSY | 1 mM [U-100% 13C; U-100% 15N] MvaT, 50 mM sodium phosphate, 50 mM sodium chloride, 1 mM DNA (5'-D(*CP*GP*CP*AP*TP*AP*TP*AP*TP*GP*CP*G)-3') | 90% H2O/10% D2O | 0.1 | 6 | ambient | 298 | |
| 3 | 3D HCCH-COSY | 1 mM [U-100% 13C; U-100% 15N] MvaT, 50 mM sodium phosphate, 50 mM sodium chloride, 1 mM DNA (5'-D(*CP*GP*CP*AP*TP*AP*TP*AP*TP*GP*CP*G)-3') | 90% H2O/10% D2O | 0.1 | 6 | ambient | 298 | |
| 4 | 3D 1H-15N NOESY | 1 mM [U-100% 13C; U-100% 15N] MvaT, 50 mM sodium phosphate, 50 mM sodium chloride, 1 mM DNA (5'-D(*CP*GP*CP*AP*TP*AP*TP*AP*TP*GP*CP*G)-3') | 90% H2O/10% D2O | 0.1 | 6 | ambient | 298 | |
| 5 | 3D 1H-13C NOESY | 1 mM [U-100% 13C; U-100% 15N] MvaT, 50 mM sodium phosphate, 50 mM sodium chloride, 1 mM DNA (5'-D(*CP*GP*CP*AP*TP*AP*TP*AP*TP*GP*CP*G)-3') | 90% H2O/10% D2O | 0.1 | 6 | ambient | 298 | |
| 6 | 2D 1H-1H NOESY | 1 mM [U-100% 13C; U-100% 15N] MvaT, 50 mM sodium phosphate, 50 mM sodium chloride, 1 mM DNA (5'-D(*CP*GP*CP*AP*TP*AP*TP*AP*TP*GP*CP*G)-3') | 90% H2O/10% D2O | 0.1 | 6 | ambient | 298 | |
| 7 | 2D 1H-1H TOCSY | 1 mM [U-100% 13C; U-100% 15N] MvaT, 50 mM sodium phosphate, 50 mM sodium chloride, 1 mM DNA (5'-D(*CP*GP*CP*AP*TP*AP*TP*AP*TP*GP*CP*G)-3') | 90% H2O/10% D2O | 0.1 | 6 | ambient | 298 | |
