NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 2D 1H-1H NOESY | 0.6 mM BPSL1050, 20 mM potassium phosphate, 150 mM sodium chloride, 2 mM DTT, 150 mM [U-100% 2H] DSS | 100% D2O | 0.15 | 6.8 | ambient | 298 | |
2 | 2D 1H-1H NOESY | 0.7 mM BPSL1050, 20 mM potassium phosphate, 150 mM sodium chloride, 2 mM DTT, 150 mM [U-100% 2H] DSS | 90% H2O/10% D2O | 0.15 | 6.8 | ambient | 298 | |
3 | 3D 1H-15N NOESY | 0.7 mM [U-99% 15N] BPSL1050, 20 mM potassium phosphate, 150 mM sodium chloride, 2 mM DTT, 150 mM [U-100% 2H] DSS | 90% H2O/10% D2O | 0.15 | 6.8 | ambient | 298 | |
4 | 3D 1H-13C NOESY | 0.7 mM [U-99% 13C; U-99% 15N] BPSL1050, 20 mM potassium phosphate, 150 mM sodium chloride, 2 mM DTT, 150 mM [U-100% 2H] DSS | 100% D2O | 0.15 | 6.8 | ambient | 298 | |
5 | HN-N Residual dipolar coupling | 0.7 mM [U-99% 15N] BPSL1050, 20 mM potassium phosphate, 150 mM sodium chloride, 2 mM DTT, 150 mM [U-100% 2H] DSS | 90% H2O/10% D2O | 0.15 | 6.8 | ambient | 298 | |