2MKF
Solution structure of the E81 deletion mutant of the tandem UIMs of RAP80
SOLUTION NMR
NMR Experiment | ||||||||
---|---|---|---|---|---|---|---|---|
Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
1 | 2D 1H-15N HSQC | 0.5-1 mM [U-100% 15N] deltaE81-RAP80-tUIM | 90% H2O/10% D2O | 150 | 7.3 | ambient | 278 | |
2 | 3D 1H-15N NOESY | 0.5-1 mM [U-100% 15N] deltaE81-RAP80-tUIM | 90% H2O/10% D2O | 150 | 7.3 | ambient | 278 | |
3 | 3D CBCA(CO)NH | 0.5-1 mM [U-100% 13C; U-100% 15N] deltaE81-RAP80-tUIM | 90% H2O/10% D2O | 150 | 7.3 | ambient | 278 | |
4 | 3D HNCACB | 0.5-1 mM [U-100% 13C; U-100% 15N] deltaE81-RAP80-tUIM | 90% H2O/10% D2O | 150 | 7.3 | ambient | 278 | |
5 | 3D HNCO | 0.5-1 mM [U-100% 13C; U-100% 15N] deltaE81-RAP80-tUIM | 90% H2O/10% D2O | 150 | 7.3 | ambient | 278 | |
6 | 3D HBHA(CO)NH | 0.5-1 mM [U-100% 13C; U-100% 15N] deltaE81-RAP80-tUIM | 90% H2O/10% D2O | 150 | 7.3 | ambient | 278 |
NMR Spectrometer Information | |||
---|---|---|---|
Spectrometer | Manufacturer | Model | Field Strength |
1 | Varian | INOVA | 600 |
NMR Ensemble Information | |
---|---|
Conformer Selection Criteria | all calculated structures submitted |
Conformers Calculated Total Number | 20 |
Conformers Submitted Total Number | 20 |
Representative Model | 1 (closest to the average) |
Computation: NMR Software | ||||
---|---|---|---|---|
# | Classification | Version | Software Name | Author |
1 | structure solution | X-PLOR NIH | Schwieters, Kuszewski, Tjandra and Clore | |
2 | collection | VnmrJ | Varian | |
3 | processing | NMRPipe | Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax | |
4 | chemical shift assignment | CARA | Keller and Wuthrich | |
5 | refinement | X-PLOR NIH | Schwieters, Kuszewski, Tjandra and Clore |