NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 2D 1H-15N-TROSY | 0.7 mM [U-100% 15N] RLIP76-GAP-GBD, 20 mM TRIS, 100 mM sodium chloride, 0.05 % sodium azide, 5 mM DTT, 90 % H20, 10 % D20 | 90% H2O/10% D2O | | 7.9 | 1 atm | 298 | |
2 | 3D HNCA | 0.4 mM [U-100% 13C; U-100% 15N; U-80% 2H] RLIP76-GAP-GBD, 20 mM TRIS, 100 mM sodium chloride, 0.05 % sodium azide, 5 mM DTT, 90 % H20, 10 % D20 | 90% H2O/10% D2O | | 7.9 | 1 atm | 298 | |
3 | 3D HN(CO)CA | 0.4 mM [U-100% 13C; U-100% 15N; U-80% 2H] RLIP76-GAP-GBD, 20 mM TRIS, 100 mM sodium chloride, 0.05 % sodium azide, 5 mM DTT, 90 % H20, 10 % D20 | 90% H2O/10% D2O | | 7.9 | 1 atm | 298 | |
4 | 3D HNCACB | 0.4 mM [U-100% 13C; U-100% 15N; U-80% 2H] RLIP76-GAP-GBD, 20 mM TRIS, 100 mM sodium chloride, 0.05 % sodium azide, 5 mM DTT, 90 % H20, 10 % D20 | 90% H2O/10% D2O | | 7.9 | 1 atm | 298 | |
5 | 3D HN(COCA)CB | 0.4 mM [U-100% 13C; U-100% 15N; U-80% 2H] RLIP76-GAP-GBD, 20 mM TRIS, 100 mM sodium chloride, 0.05 % sodium azide, 5 mM DTT, 90 % H20, 10 % D20 | 90% H2O/10% D2O | | 7.9 | 1 atm | 298 | |
6 | 3D HNCO | 0.4 mM [U-100% 13C; U-100% 15N; U-80% 2H] RLIP76-GAP-GBD, 20 mM TRIS, 100 mM sodium chloride, 0.05 % sodium azide, 5 mM DTT, 90 % H20, 10 % D20 | 90% H2O/10% D2O | | 7.9 | 1 atm | 298 | |
7 | 3D 1H-15N NOESY | 0.7 mM [U-100% 15N] RLIP76-GAP-GBD, 20 mM TRIS, 100 mM sodium chloride, 0.05 % sodium azide, 5 mM DTT, 90 % H20, 10 % D20 | 90% H2O/10% D2O | | 7.9 | 1 atm | 298 | |
8 | 3D HBHA(CBCACO)NH | 0.6 mM [U-100% 13C; U-100% 15N] RLIP76-GAP-GBD, 20 mM TRIS, 100 mM sodium chloride, 0.05 % sodium azide, 5 mM DTT, 90 % H20, 10 % D20 | 90% H2O/10% D2O | | 7.9 | 1 atm | 298 | |
9 | 3D HCCH-TOCSY | 0.6 mM [U-100% 13C; U-100% 15N] RLIP76-GAP-GBD, 20 mM TRIS, 100 mM sodium chloride, 0.05 % sodium azide, 5 mM DTT, 90 % H20, 10 % D20 | 90% H2O/10% D2O | | 7.9 | 1 atm | 298 | |
10 | 3D HCCH-TOCSY | 0.6 mM [U-100% 13C; U-100% 15N] RLIP76-GAP-GBD, 20 mM TRIS, 100 mM sodium chloride, 0.05 % sodium azide, 5 mM DTT, 90 % H20, 10 % D20 | 90% H2O/10% D2O | | 7.9 | 1 atm | 298 | |
11 | 3D 1H-13C NOESY | 0.6 mM [U-100% 13C; U-100% 15N] RLIP76-GAP-GBD, 20 mM TRIS, 100 mM sodium chloride, 0.05 % sodium azide, 5 mM DTT, 90 % H20, 10 % D20 | 90% H2O/10% D2O | | 7.9 | 1 atm | 298 | |
12 | 2D 1H-13C HSQC | 0.6 mM [U-100% 13C; U-100% 15N] RLIP76-GAP-GBD, 20 mM TRIS, 100 mM sodium chloride, 0.05 % sodium azide, 5 mM DTT, 90 % H20, 10 % D20 | 90% H2O/10% D2O | | 7.9 | 1 atm | 298 | |
13 | 3D HCCH-COSY | 0.6 mM [U-100% 13C; U-100% 15N] RLIP76-GAP-GBD, 20 mM TRIS, 100 mM sodium chloride, 0.05 % sodium azide, 5 mM DTT, 90 % H20, 10 % D20 | 90% H2O/10% D2O | | 7.9 | 1 atm | 298 | |