NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 2D 1H-15N HSQC | 1 mM [U-98% 13C; U-98% 15N] PSIAA4, 50 mM sodium phosphate, 100 mM sodium chloride, 2 mM magnesium chloride, 1 mM DTT | 90% H2O/10% D2O | 0.112 | 2.5 | ambient | 298 | |
2 | 2D 1H-13C HSQC aliphatic | 1 mM [U-98% 13C; U-98% 15N] PSIAA4, 50 mM sodium phosphate, 100 mM sodium chloride, 2 mM magnesium chloride, 1 mM DTT | 90% H2O/10% D2O | 0.112 | 2.5 | ambient | 298 | |
3 | 2D 1H-13C HSQC aromatic | 1 mM [U-98% 13C; U-98% 15N] PSIAA4, 50 mM sodium phosphate, 100 mM sodium chloride, 2 mM magnesium chloride, 1 mM DTT | 90% H2O/10% D2O | 0.112 | 2.5 | ambient | 298 | |
4 | 3D HNCA | 1 mM [U-98% 13C; U-98% 15N] PSIAA4, 50 mM sodium phosphate, 100 mM sodium chloride, 2 mM magnesium chloride, 1 mM DTT | 90% H2O/10% D2O | 0.112 | 2.5 | ambient | 298 | |
5 | 3D HNCACB | 1 mM [U-98% 13C; U-98% 15N] PSIAA4, 50 mM sodium phosphate, 100 mM sodium chloride, 2 mM magnesium chloride, 1 mM DTT | 90% H2O/10% D2O | 0.112 | 2.5 | ambient | 298 | |
6 | 3D HN(COCA)CB | 1 mM [U-98% 13C; U-98% 15N] PSIAA4, 50 mM sodium phosphate, 100 mM sodium chloride, 2 mM magnesium chloride, 1 mM DTT | 90% H2O/10% D2O | 0.112 | 2.5 | ambient | 298 | |
7 | 3D 1H-15N NOESY | 1 mM [U-98% 13C; U-98% 15N] PSIAA4, 50 mM sodium phosphate, 100 mM sodium chloride, 2 mM magnesium chloride, 1 mM DTT | 90% H2O/10% D2O | 0.112 | 2.5 | ambient | 298 | |
8 | 3D 1H-13C NOESY aliphatic | 1 mM [U-98% 13C; U-98% 15N] PSIAA4, 50 mM sodium phosphate, 100 mM sodium chloride, 2 mM magnesium chloride, 1 mM DTT | 90% H2O/10% D2O | 0.112 | 2.5 | ambient | 298 | |
9 | 3D 1H-13C NOESY aromatic | 1 mM [U-98% 13C; U-98% 15N] PSIAA4, 50 mM sodium phosphate, 100 mM sodium chloride, 2 mM magnesium chloride, 1 mM DTT | 90% H2O/10% D2O | 0.112 | 2.5 | ambient | 298 | |
10 | 2D 1H-1H NOESY | 1 mM [U-98% 13C; U-98% 15N] PSIAA4, 50 mM sodium phosphate, 100 mM sodium chloride, 2 mM magnesium chloride, 1 mM DTT | 90% H2O/10% D2O | 0.112 | 2.5 | ambient | 298 | |
11 | 3D HNCO | 1 mM [U-98% 13C; U-98% 15N] PSIAA4, 50 mM sodium phosphate, 100 mM sodium chloride, 2 mM magnesium chloride, 1 mM DTT | 90% H2O/10% D2O | 0.112 | 2.5 | ambient | 298 | |
12 | 3D HCCH-TOCSY | 1 mM [U-98% 13C; U-98% 15N] PSIAA4, 50 mM sodium phosphate, 100 mM sodium chloride, 2 mM magnesium chloride, 1 mM DTT | 90% H2O/10% D2O | 0.112 | 2.5 | ambient | 298 | |