| NMR Experiment |
|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
|---|
| 1 | 2D 1H-15N HSQC | 400 uM [U-99% 13C; U-99% 15N] PDZ2, 20 mM TRIS, 150 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, 688 uM CFTR | 90% H2O/10% D2O | 150 | 7.5 | ambient | 288 | |
| 2 | 2D 1H-13C HSQC aliphatic | 400 uM [U-99% 13C; U-99% 15N] PDZ2, 20 mM TRIS, 150 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, 688 uM CFTR | 90% H2O/10% D2O | 150 | 7.5 | ambient | 288 | |
| 3 | 2D 1H-13C HSQC aromatic | 400 uM [U-99% 13C; U-99% 15N] PDZ2, 20 mM TRIS, 150 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, 688 uM CFTR | 90% H2O/10% D2O | 150 | 7.5 | ambient | 288 | |
| 4 | 3D 1H-15N NOESY | 400 uM [U-99% 13C; U-99% 15N] PDZ2, 20 mM TRIS, 150 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, 688 uM CFTR | 90% H2O/10% D2O | 150 | 7.5 | ambient | 288 | |
| 5 | 3D 1H-13C NOESY aliphatic | 400 uM [U-99% 13C; U-99% 15N] PDZ2, 20 mM TRIS, 150 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, 688 uM CFTR | 90% H2O/10% D2O | 150 | 7.5 | ambient | 288 | |
| 6 | 3D 1H-13C NOESY aromatic | 400 uM [U-99% 13C; U-99% 15N] PDZ2, 20 mM TRIS, 150 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, 688 uM CFTR | 90% H2O/10% D2O | 150 | 7.5 | ambient | 288 | |
| 7 | 3D HNCA | 400 uM [U-99% 13C; U-99% 15N] PDZ2, 20 mM TRIS, 150 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, 688 uM CFTR | 90% H2O/10% D2O | 150 | 7.5 | ambient | 288 | |
| 8 | 3D CBCA(CO)NH | 400 uM [U-99% 13C; U-99% 15N] PDZ2, 20 mM TRIS, 150 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, 688 uM CFTR | 90% H2O/10% D2O | 150 | 7.5 | ambient | 288 | |
| 9 | 3D HBHA(CO)NH | 400 uM [U-99% 13C; U-99% 15N] PDZ2, 20 mM TRIS, 150 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, 688 uM CFTR | 90% H2O/10% D2O | 150 | 7.5 | ambient | 288 | |
| 10 | 3D HNCACB | 400 uM [U-99% 13C; U-99% 15N] PDZ2, 20 mM TRIS, 150 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, 688 uM CFTR | 90% H2O/10% D2O | 150 | 7.5 | ambient | 288 | |
| 11 | 3D HNCO | 400 uM [U-99% 13C; U-99% 15N] PDZ2, 20 mM TRIS, 150 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, 688 uM CFTR | 90% H2O/10% D2O | 150 | 7.5 | ambient | 288 | |
| 12 | 3D H(CCO)NH | 400 uM [U-99% 13C; U-99% 15N] PDZ2, 20 mM TRIS, 150 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, 688 uM CFTR | 90% H2O/10% D2O | 150 | 7.5 | ambient | 288 | |
| 13 | 3D HCCH-TOCSY | 200 uM [U-99% 13C; U-99% 15N] PDZ2, 20 mM TRIS, 150 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, 275 uM CFTR | 90% H2O/10% D2O | 150 | 7.5 | ambient | 288 | |
| 14 | 2D 13C,15N f1,f2-filtered TOCSY | 400 uM [U-99% 13C; U-99% 15N] PDZ2, 20 mM TRIS, 150 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, 688 uM CFTR | 90% H2O/10% D2O | 150 | 7.5 | ambient | 288 | |
| 15 | 2D 13C,15N f1,f2-filtered NOESY | 400 uM [U-99% 13C; U-99% 15N] PDZ2, 20 mM TRIS, 150 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, 688 uM CFTR | 90% H2O/10% D2O | 150 | 7.5 | ambient | 288 | |
| 16 | 2D 13C,15N f2-filtered NOESY | 400 uM [U-99% 13C; U-99% 15N] PDZ2, 20 mM TRIS, 150 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, 688 uM CFTR | 90% H2O/10% D2O | 150 | 7.5 | ambient | 288 | |
| 17 | 3D 13C,15N f1-filtered 13C-edited NOESY-HSQC | 400 uM [U-99% 13C; U-99% 15N] PDZ2, 20 mM TRIS, 150 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, 688 uM CFTR | 90% H2O/10% D2O | 150 | 7.5 | ambient | 288 | |
| 18 | 3D 13C,15N f1-filtered 15N-edited NOESY-HSQC | 400 uM [U-99% 13C; U-99% 15N] PDZ2, 20 mM TRIS, 150 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, 688 uM CFTR | 90% H2O/10% D2O | 150 | 7.5 | ambient | 288 | |
