| NMR Experiment |
|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
|---|
| 1 | 2D 1H-15N HSQC | 1.7 mM [U-99% 13C; U-99% 15N] protein_1, 50 mM sodium phosphate, 10 mM beta-mercaptoethanol, 150 mM sodium chloride | 90% H2O/10% D2O | 150 | 8 | ambient | 293.15 | |
| 2 | 3D HBHA(CO)NH | 1.7 mM [U-99% 13C; U-99% 15N] protein_1, 50 mM sodium phosphate, 10 mM beta-mercaptoethanol, 150 mM sodium chloride | 90% H2O/10% D2O | 150 | 8 | ambient | 293.15 | |
| 3 | 3D HNCA | 1.7 mM [U-99% 13C; U-99% 15N] protein_1, 50 mM sodium phosphate, 10 mM beta-mercaptoethanol, 150 mM sodium chloride | 90% H2O/10% D2O | 150 | 8 | ambient | 293.15 | |
| 4 | 3D CBCA(CO)NH | 1.7 mM [U-99% 13C; U-99% 15N] protein_1, 50 mM sodium phosphate, 10 mM beta-mercaptoethanol, 150 mM sodium chloride | 90% H2O/10% D2O | 150 | 8 | ambient | 293.15 | |
| 5 | 3D 1H-13C NOESY aliphatic | 1.7 mM [U-99% 13C; U-99% 15N] protein_1, 50 mM sodium phosphate, 10 mM beta-mercaptoethanol, 150 mM sodium chloride | 90% H2O/10% D2O | 150 | 8 | ambient | 293.15 | |
| 6 | 3D 1H-13C NOESY aromatic | 1.7 mM [U-99% 13C; U-99% 15N] protein_1, 50 mM sodium phosphate, 10 mM beta-mercaptoethanol, 150 mM sodium chloride | 90% H2O/10% D2O | 150 | 8 | ambient | 293.15 | |
