2LQZ
Structure of the RNA claw of the DNA packaging motor of bacteriophage 29
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 2D 1H-1H NOESY | 300 uM [U-100% 13C; U-100% 15N] RNA (27-MER) | 90% H2O/10% D2O | 50 | 6.5 | ambient | 298 | |
| 2 | 2D 1H-15N HSQC | 300 uM [U-100% 13C; U-100% 15N] RNA (27-MER) | 90% H2O/10% D2O | 50 | 6.5 | ambient | 298 | |
| 3 | 2D 1H-13C HSQC aliphatic | 300 uM [U-100% 13C; U-100% 15N] RNA (27-MER) | 90% H2O/10% D2O | 50 | 6.5 | ambient | 298 | |
| 4 | 2D 1H-13C HSQC aromatic | 300 uM [U-100% 13C; U-100% 15N] RNA (27-MER) | 90% H2O/10% D2O | 50 | 6.5 | ambient | 298 | |
| 5 | 2D DQF-COSY | 300 uM [U-100% 13C; U-100% 15N] RNA (27-MER) | 90% H2O/10% D2O | 50 | 6.5 | ambient | 298 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | INOVA | 800 |
| 2 | Varian | INOVA | 600 |
| 3 | Varian | INOVA | 900 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| simulated annealing | CNS | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the lowest energy |
| Conformers Calculated Total Number | 50 |
| Conformers Submitted Total Number | 9 |
| Representative Model | 1 (closest to the average) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | CNS | Brunger A. T. et.al. | |
