NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 2D 1H-15N HSQC | 2 mM [U-100% 13C; U-100% 15N] Nrd1 polypeptide, 2.3 mM phosphopeptide | 90% H2O/10% D2O | 100 | 8 | ambient | 293 | |
2 | 2D 1H-13C HSQC | 2 mM [U-100% 13C; U-100% 15N] Nrd1 polypeptide, 2.3 mM phosphopeptide | 90% H2O/10% D2O | 100 | 8 | ambient | 293 | |
3 | 3D CBCA(CO)NH | 2 mM [U-100% 13C; U-100% 15N] Nrd1 polypeptide, 2.3 mM phosphopeptide | 90% H2O/10% D2O | 100 | 8 | ambient | 293 | |
4 | 3D HNCA | 2 mM [U-100% 13C; U-100% 15N] Nrd1 polypeptide, 2.3 mM phosphopeptide | 90% H2O/10% D2O | 100 | 8 | ambient | 293 | |
5 | 3D HNCACB | 2 mM [U-100% 13C; U-100% 15N] Nrd1 polypeptide, 2.3 mM phosphopeptide | 90% H2O/10% D2O | 100 | 8 | ambient | 293 | |
6 | 3D HN(CO)CA | 2 mM [U-100% 13C; U-100% 15N] Nrd1 polypeptide, 2.3 mM phosphopeptide | 90% H2O/10% D2O | 100 | 8 | ambient | 293 | |
7 | 3D HCCH-TOCSY | 2 mM [U-100% 13C; U-100% 15N] Nrd1 polypeptide, 2.3 mM phosphopeptide | 90% H2O/10% D2O | 100 | 8 | ambient | 293 | |
8 | 3D 1H-15N NOESY | 2 mM [U-100% 13C; U-100% 15N] Nrd1 polypeptide, 2.3 mM phosphopeptide | 90% H2O/10% D2O | 100 | 8 | ambient | 293 | |
9 | 3D 1H-13C NOESY aliphatic | 2 mM [U-100% 13C; U-100% 15N] Nrd1 polypeptide, 2.3 mM phosphopeptide | 90% H2O/10% D2O | 100 | 8 | ambient | 293 | |
10 | 3D filtered-edited NOESY | 2 mM [U-100% 13C; U-100% 15N] Nrd1 polypeptide, 2.3 mM phosphopeptide | 90% H2O/10% D2O | 100 | 8 | ambient | 293 | |
11 | 2D filtered-filtered NOESY | 2 mM [U-100% 13C; U-100% 15N] Nrd1 polypeptide, 2.3 mM phosphopeptide | 90% H2O/10% D2O | 100 | 8 | ambient | 293 | |