2LM7
NMR structure of the C-terminal domain of VP7 in membrane mimicking micelles
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 2D 1H-1H NOESY | 1 mM VP7-61-1, 100 mM [U-100% 2H] DPC-2 | 95% H2O/5% D2O | 0 | 3 | ambient | 323 | |
| 2 | 2D 1H-1H TOCSY | 1 mM VP7-61-1, 100 mM [U-100% 2H] DPC-2 | 95% H2O/5% D2O | 0 | 3 | ambient | 323 | |
| 3 | 2D DQF-COSY | 1 mM VP7-61-1, 100 mM [U-100% 2H] DPC-2 | 95% H2O/5% D2O | 0 | 3 | ambient | 323 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | AVANCE | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| DGSA-distance geometry simulated annealing | ARIA | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the lowest energy |
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 8 |
| Representative Model | 1 (lowest energy) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | structure solution | ARIA | 2.3 | Linge, O'Donoghue and Nilges |
| 2 | refinement | ARIA | 2.3 | Linge, O'Donoghue and Nilges |
