2LC8
Solution structure of the MLV readthrough pseudoknot
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 2D 1H-1H NOESY | 0.2-2.0 mM RNA (59-MER) | 100% D2O | 0.02 | 7.4 | ambient | 298 | |
| 2 | 2D 1H-13C HSQC | 0.2-2.0 mM [U-100% 13C; U-100% 15N] RNA (59-MER) | 100% D2O | 0.02 | 7.4 | ambient | 298 | |
| 3 | 2D 1H-13C HSQC | 0.2-2.0 mM [U-100% 2H] RNA (59-MER) | 100% D2O | 0.02 | 7.4 | ambient | 298 | |
| 4 | 2D 1H-1H NOESY | 0.2-2.0 mM RNA (59-MER) | 100% D2O | 0.02 | 7.4 | ambient | 298 | |
| 5 | 2D 1H-1H NOESY | 0.2-2.0 mM [U-100% 2H] RNA (59-MER) | 100% D2O | 0.02 | 7.4 | ambient | 298 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | AVANCE | 700 |
| 2 | Bruker | AVANCE | 900 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| simulated annealing | CYANA | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the least restraint violations |
| Conformers Calculated Total Number | 200 |
| Conformers Submitted Total Number | 10 |
| Representative Model | 1 (lowest energy) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | structure solution | CYANA | Guntert, Mumenthaler and Wuthrich | |
| 2 | refinement | CYANA | Guntert, Mumenthaler and Wuthrich | |
