2L3J
The solution structure of the ADAR2 dsRBM-RNA complex reveals a sequence-specific read out of the minor groove
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 2D 1H-15N HSQC | 1 mM [U-100% 15N] entity_1-1, 1 mM RNA (71-MER)-2 | 90% H2O/10% D2O | 0 | 7.6 | ambient | 310 | |
| 2 | 2D 1H-15N TROSY temp compensated | 1 mM [U-100% 15N] entity_1-1, 1 mM RNA (71-MER)-2 | 90% H2O/10% D2O | 0 | 7.6 | ambient | 310 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | AVANCE | 900 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| simulated annealing | TopSpin | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the lowest energy |
| Conformers Calculated Total Number | 40 |
| Conformers Submitted Total Number | 19 |
| Representative Model | 1 (lowest energy) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | collection | TopSpin | Bruker Biospin | |
| 2 | data analysis | Sparky | Goddard | |
| 3 | structure solution | CYANA | Guntert, Mumenthaler and Wuthrich | |
| 4 | refinement | Amber | Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo and Kollm | |
