NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 2D 1H-15N HSQC | 0.5 mM [U-100% 13C; U-100% 15N] E. coli protein, 15 % D2O, 85 % H2O, 20 mM Na2HPO4/NaH2PO4 | 85% H2O/15% D2O | 0.02 | 6.6 | ambient | 288 | |
2 | 3D CBCA(CO)NH | 0.5 mM [U-100% 13C; U-100% 15N] E. coli protein, 15 % D2O, 85 % H2O, 20 mM Na2HPO4/NaH2PO4 | 85% H2O/15% D2O | 0.02 | 6.6 | ambient | 288 | |
3 | 3D 1H-15N NOESY | 0.5 mM [U-100% 13C; U-100% 15N] E. coli protein, 15 % D2O, 85 % H2O, 20 mM Na2HPO4/NaH2PO4 | 85% H2O/15% D2O | 0.02 | 6.6 | ambient | 288 | |
4 | 3D HNCO | 0.5 mM [U-100% 13C; U-100% 15N] E. coli protein, 15 % D2O, 85 % H2O, 20 mM Na2HPO4/NaH2PO4 | 85% H2O/15% D2O | 0.02 | 6.6 | ambient | 288 | |
5 | 3D HNCACB | 0.5 mM [U-100% 13C; U-100% 15N] E. coli protein, 15 % D2O, 85 % H2O, 20 mM Na2HPO4/NaH2PO4 | 85% H2O/15% D2O | 0.02 | 6.6 | ambient | 288 | |
6 | 3D HN(CO)CA | 0.5 mM [U-100% 13C; U-100% 15N] E. coli protein, 15 % D2O, 85 % H2O, 20 mM Na2HPO4/NaH2PO4 | 85% H2O/15% D2O | 0.02 | 6.6 | ambient | 288 | |
7 | 3D 1H-13C NOESY | 0.5 mM [U-100% 13C; U-100% 15N] E. coli protein, 15 % D2O, 85 % H2O, 20 mM Na2HPO4/NaH2PO4 | 85% H2O/15% D2O | 0.02 | 6.6 | ambient | 288 | |
8 | 2D 1H-1H COSY | 0.5 mM [U-100% 13C; U-100% 15N] E. coli protein, 15 % D2O, 85 % H2O, 20 mM Na2HPO4/NaH2PO4 | 85% H2O/15% D2O | 0.02 | 6.6 | ambient | 288 | |