NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 2D 1H-15N HSQC | 1 mM [U-100% 15N] protein-1, 1 mM DTT-2, 50 mM TRIS-3, 1 mM EDTA-4 | 90% H2O/10% D2O | 0.1 | 7 | ambient | 308 | |
2 | 2D 1H-13C HSQC | 1 mM [U-100% 13C; U-100% 15N] protein-5, 50 mM [U-100% 2H] TRIS-6, 1 mM [U-100% 2H] DTT-7, 1 mM EDTA-8 | 100% D2O | 0.1 | 7 | ambient | 308 | |
3 | 3D HNCACB | 1 mM [U-100% 13C; U-100% 15N] protein-9, 1 mM DTT-10, 50 mM TRIS-11, 1 mM EDTA-12 | 90% H2O/10% D2O | 0.1 | 7 | ambient | 308 | |
4 | 3D CBCA(CO)NH | 1 mM [U-100% 13C; U-100% 15N] protein-9, 1 mM DTT-10, 50 mM TRIS-11, 1 mM EDTA-12 | 90% H2O/10% D2O | 0.1 | 7 | ambient | 308 | |
5 | 2D 1H-1H NOESY | 1 mM protein-13, 1 mM [U-100% 2H] DTT-14, 50 mM [U-100% 2H] TRIS-15, 1 mM EDTA-16 | 100% D2O | 0.1 | 7 | ambient | 308 | |
6 | 3D 1H-15N NOESY | 1 mM [U-100% 15N] protein-1, 1 mM DTT-2, 50 mM TRIS-3, 1 mM EDTA-4 | 90% H2O/10% D2O | 0.1 | 7 | ambient | 308 | |
7 | 3D 1H-13C NOESY | 1 mM [U-100% 13C; U-100% 15N] protein-5, 50 mM [U-100% 2H] TRIS-6, 1 mM [U-100% 2H] DTT-7, 1 mM EDTA-8 | 100% D2O | 0.1 | 7 | ambient | 308 | |