2KX8

NMR structure of stem-loop 4 from the human 7SK snRNA in complex with arginine

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	2D 1H-1H NOESY	1 mM 7SK-1	90% H2O/10% D2O	0.02	5.2	ambient	301
2	2D 1H-13C HSQC	2 mM [U-13C; U-15N]-Ade/Ura 7SK-AU-2	100% D2O	0.02	5.2	ambient	301
3	3D 1H-13C NOESY	2 mM [U-13C; U-15N]-Ade/Ura 7SK-AU-2	100% D2O	0.02	5.2	ambient	301
4	2D 1H-13C HSQC	2 mM [U-13C; U-15N]-Gua/Cyt 7SK-GC-3	100% D2O	0.02	5.2	ambient	301
5	3D 1H-13C NOESY	2 mM [U-13C; U-15N]-Gua/Cyt 7SK-GC-3	100% D2O	0.02	5.2	ambient	301

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	DMX	700

NMR Refinement
Method	Details	Software
torsion angle dynamics		CYANA

NMR Ensemble Information
Conformer Selection Criteria	structures with the least restraint violations
Conformers Calculated Total Number	200
Conformers Submitted Total Number	10
Representative Model	1 (lowest energy)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	structure solution	CYANA		Guntert, Mumenthaler and Wuthrich
2	refinement	CYANA		Guntert, Mumenthaler and Wuthrich

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.