NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 2D 1H-15N HSQC | 1 mM [U-100% 13C; U-100% 15N] protein, 3.5 mM peptide, 20 mM sodium phosphate, 30 mM sodium chloride, 0.001 % sodium azide | 90% H2O/10% D2O | 0.08 | 7.0 | ambient | 301 | |
2 | 2D 1H-15N HSQC | 2.5 mM protein, 0.5 mM [U-100% 13C; U-100% 15N] peptide, 20 mM sodium phosphate, 30 mM sodium chloride, 0.001 % sodium azide | 90% H2O/10% D2O | 0.08 | 7.0 | ambient | 301 | |
3 | 2D 1H-13C HSQC | 1 mM [U-100% 13C; U-100% 15N] protein, 3.5 mM peptide, 20 mM sodium phosphate, 30 mM sodium chloride, 0.001 % sodium azide | 90% H2O/10% D2O | 0.08 | 7.0 | ambient | 301 | |
4 | 2D 1H-13C HSQC | 2.5 mM protein, 0.5 mM [U-100% 13C; U-100% 15N] peptide, 20 mM sodium phosphate, 30 mM sodium chloride, 0.001 % sodium azide | 90% H2O/10% D2O | 0.08 | 7.0 | ambient | 301 | |
5 | 3D HNCACB | 1 mM [U-100% 13C; U-100% 15N] protein, 3.5 mM peptide, 20 mM sodium phosphate, 30 mM sodium chloride, 0.001 % sodium azide | 90% H2O/10% D2O | 0.08 | 7.0 | ambient | 301 | |
6 | 3D HNCA | 2.5 mM protein, 0.5 mM [U-100% 13C; U-100% 15N] peptide, 20 mM sodium phosphate, 30 mM sodium chloride, 0.001 % sodium azide | 90% H2O/10% D2O | 0.08 | 7.0 | ambient | 301 | |
7 | 3D CBCA(CO)NH | 1 mM [U-100% 13C; U-100% 15N] protein, 3.5 mM peptide, 20 mM sodium phosphate, 30 mM sodium chloride, 0.001 % sodium azide | 90% H2O/10% D2O | 0.08 | 7.0 | ambient | 301 | |
8 | 3D CBCA(CO)NH | 2.5 mM protein, 0.5 mM [U-100% 13C; U-100% 15N] peptide, 20 mM sodium phosphate, 30 mM sodium chloride, 0.001 % sodium azide | 90% H2O/10% D2O | 0.08 | 7.0 | ambient | 301 | |
9 | 3D HNCO | 1 mM [U-100% 13C; U-100% 15N] protein, 3.5 mM peptide, 20 mM sodium phosphate, 30 mM sodium chloride, 0.001 % sodium azide | 90% H2O/10% D2O | 0.08 | 7.0 | ambient | 301 | |
10 | 3D HNCO | 2.5 mM protein, 0.5 mM [U-100% 13C; U-100% 15N] peptide, 20 mM sodium phosphate, 30 mM sodium chloride, 0.001 % sodium azide | 90% H2O/10% D2O | 0.08 | 7.0 | ambient | 301 | |
11 | 3D HCACO | 2.5 mM protein, 0.5 mM [U-100% 13C; U-100% 15N] peptide, 20 mM sodium phosphate, 30 mM sodium chloride, 0.001 % sodium azide | 90% H2O/10% D2O | 0.08 | 7.0 | ambient | 301 | |
12 | 3D C(CO)NH | 1 mM [U-100% 13C; U-100% 15N] protein, 3.5 mM peptide, 20 mM sodium phosphate, 30 mM sodium chloride, 0.001 % sodium azide | 90% H2O/10% D2O | 0.08 | 7.0 | ambient | 301 | |
13 | 3D H(CCO)NH | 1 mM [U-100% 13C; U-100% 15N] protein, 3.5 mM peptide, 20 mM sodium phosphate, 30 mM sodium chloride, 0.001 % sodium azide | 90% H2O/10% D2O | 0.08 | 7.0 | ambient | 301 | |
14 | 3D HCCH-TOCSY | 1 mM [U-100% 13C; U-100% 15N] protein, 3.5 mM peptide, 20 mM sodium phosphate, 30 mM sodium chloride, 0.001 % sodium azide | 90% H2O/10% D2O | 0.08 | 7.0 | ambient | 301 | |
15 | 3D HCCH-TOCSY | 2.5 mM protein, 0.5 mM [U-100% 13C; U-100% 15N] peptide, 20 mM sodium phosphate, 30 mM sodium chloride, 0.001 % sodium azide | 90% H2O/10% D2O | 0.08 | 7.0 | ambient | 301 | |
16 | 3D 1H-15N NOESY | 1 mM [U-100% 13C; U-100% 15N] protein, 3.5 mM peptide, 20 mM sodium phosphate, 30 mM sodium chloride, 0.001 % sodium azide | 90% H2O/10% D2O | 0.08 | 7.0 | ambient | 301 | |
17 | 3D 1H-15N NOESY | 2.5 mM protein, 0.5 mM [U-100% 13C; U-100% 15N] peptide, 20 mM sodium phosphate, 30 mM sodium chloride, 0.001 % sodium azide | 90% H2O/10% D2O | 0.08 | 7.0 | ambient | 301 | |
18 | 3D 1H-13C NOESY | 1 mM [U-100% 13C; U-100% 15N] protein, 3.5 mM peptide, 20 mM sodium phosphate, 30 mM sodium chloride, 0.001 % sodium azide | 90% H2O/10% D2O | 0.08 | 7.0 | ambient | 301 | |
19 | 3D 1H-13C NOESY | 2.5 mM protein, 0.5 mM [U-100% 13C; U-100% 15N] peptide, 20 mM sodium phosphate, 30 mM sodium chloride, 0.001 % sodium azide | 90% H2O/10% D2O | 0.08 | 7.0 | ambient | 301 | |
20 | 3D HBHA(CO)NH | 1 mM [U-100% 13C; U-100% 15N] protein, 3.5 mM peptide, 20 mM sodium phosphate, 30 mM sodium chloride, 0.001 % sodium azide | 90% H2O/10% D2O | 0.08 | 7.0 | ambient | 301 | |
21 | 3D HBHA(CO)NH | 2.5 mM protein, 0.5 mM [U-100% 13C; U-100% 15N] peptide, 20 mM sodium phosphate, 30 mM sodium chloride, 0.001 % sodium azide | 90% H2O/10% D2O | 0.08 | 7.0 | ambient | 301 | |
22 | 3D 13C edited double-filtered | 1 mM [U-100% 13C; U-100% 15N] protein, 3.5 mM peptide, 20 mM sodium phosphate, 30 mM sodium chloride, 0.001 % sodium azide | 90% H2O/10% D2O | 0.08 | 7.0 | ambient | 301 | |
23 | 3D 13C edited double-filtered | 2.5 mM protein, 0.5 mM [U-100% 13C; U-100% 15N] peptide, 20 mM sodium phosphate, 30 mM sodium chloride, 0.001 % sodium azide | 90% H2O/10% D2O | 0.08 | 7.0 | ambient | 301 | |