NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 2D 1H-15N HSQC | 0.65 mM [U-100% 13C; U-100% 15N] UUCG-TAR-1, 5.2 mM Pyrimidinylpeptide-2, 50 mM potassium chloride-3, 25 mM potassium phosphate-4 | 90% H2O/10% D2O | 75 | 6.2 | ambient | 283 | |
2 | 2D 1H-13C HSQC | 0.65 mM [U-100% 13C; U-100% 15N] UUCG-TAR-5, 5.2 mM Pyrimidinylpeptide-6, 50 mM potassium chloride-7, 25 mM potassium phosphate-8 | 100% D2O | 75 | 6.2 | ambient | 298 | |
3 | 2D 1H-1H TOCSY | 0.65 mM UUCG-TAR-9, 5.2 mM Pyrimidinylpeptide-10, 50 mM potassium chloride-11, 25 mM potassium phosphate-12, 90% H2O / 10% D2O | 90% H2O/10% D2O | 75 | 6.2 | ambient | 298 | |
4 | 2D 1H-1H NOESY | 0.65 mM UUCG-TAR-9, 5.2 mM Pyrimidinylpeptide-10, 50 mM potassium chloride-11, 25 mM potassium phosphate-12, 90% H2O / 10% D2O | 90% H2O/10% D2O | 75 | 6.2 | ambient | 298 | |
5 | 2D 1H-1H NOESY | 0.65 mM UUCG-TAR-13, 5.2 mM Pyrimidinylpeptide-14, 50 mM potassium chloride-15, 25 mM potassium phosphate-16 | 100% D2O | 75 | 6.2 | ambient | 298 | |
6 | 3D HCCH-TOCSY | 0.65 mM [U-100% 13C; U-100% 15N] UUCG-TAR-5, 5.2 mM Pyrimidinylpeptide-6, 50 mM potassium chloride-7, 25 mM potassium phosphate-8 | 100% D2O | 75 | 6.2 | ambient | 298 | |
7 | 3D HCCH-COSY | 0.65 mM [U-100% 13C; U-100% 15N] UUCG-TAR-5, 5.2 mM Pyrimidinylpeptide-6, 50 mM potassium chloride-7, 25 mM potassium phosphate-8 | 100% D2O | 75 | 6.2 | ambient | 298 | |
8 | 3D 1H-15N NOESY | 0.65 mM [U-100% 13C; U-100% 15N] UUCG-TAR-1, 5.2 mM Pyrimidinylpeptide-2, 50 mM potassium chloride-3, 25 mM potassium phosphate-4 | 90% H2O/10% D2O | 75 | 6.2 | ambient | 283 | |
9 | 3D 1H-13C NOESY | 0.65 mM [U-100% 13C; U-100% 15N] UUCG-TAR-5, 5.2 mM Pyrimidinylpeptide-6, 50 mM potassium chloride-7, 25 mM potassium phosphate-8 | 100% D2O | 75 | 6.2 | ambient | 298 | |
10 | 2D H(C)N | 0.65 mM [U-100% 13C; U-100% 15N] UUCG-TAR-5, 5.2 mM Pyrimidinylpeptide-6, 50 mM potassium chloride-7, 25 mM potassium phosphate-8 | 100% D2O | 75 | 6.2 | ambient | 298 | |
11 | 2D 1H-15N HSQC (2J) | 0.65 mM [U-100% 13C; U-100% 15N] UUCG-TAR-5, 5.2 mM Pyrimidinylpeptide-6, 50 mM potassium chloride-7, 25 mM potassium phosphate-8 | 100% D2O | 75 | 6.2 | ambient | 298 | |
12 | 2D 1H-13C HSQC | 0.65 mM [U-100% 13C; U-100% 15N] UUCG-TAR-5, 5.2 mM Pyrimidinylpeptide-6, 50 mM potassium chloride-7, 25 mM potassium phosphate-8 | 100% D2O | 75 | 6.2 | ambient | 298 | |
13 | 2D HNN-COSY | 0.65 mM [U-100% 13C; U-100% 15N] UUCG-TAR-1, 5.2 mM Pyrimidinylpeptide-2, 50 mM potassium chloride-3, 25 mM potassium phosphate-4 | 90% H2O/10% D2O | 75 | 6.2 | ambient | 283 | |
14 | 2D H5NN-COSY | 0.65 mM [U-100% 13C; U-100% 15N] UUCG-TAR-5, 5.2 mM Pyrimidinylpeptide-6, 50 mM potassium chloride-7, 25 mM potassium phosphate-8 | 100% D2O | 75 | 6.2 | ambient | 298 | |
15 | 3D forward-directed HCC-TOCSY-CCH E.COSY | 0.65 mM [U-100% 13C; U-100% 15N] UUCG-TAR-5, 5.2 mM Pyrimidinylpeptide-6, 50 mM potassium chloride-7, 25 mM potassium phosphate-8 | 100% D2O | 75 | 6.2 | ambient | 298 | |
16 | 2D 1H-15N CPMG-NOESY | 0.65 mM [U-100% 13C; U-100% 15N] UUCG-TAR-1, 5.2 mM Pyrimidinylpeptide-2, 50 mM potassium chloride-3, 25 mM potassium phosphate-4 | 90% H2O/10% D2O | 75 | 6.2 | ambient | 283 | |