NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 1D 15N T1 | 1 mM [U-100% 13C; U-100% 15N] nsr437a, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 50 uM DSS, 0.02 % sodium azide | 90% H2O/10% D2O | 200 | 6.5 | ambient | 298 | |
2 | 1D 15N T2 | 1 mM [U-100% 13C; U-100% 15N] nsr437a, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 50 uM DSS, 0.02 % sodium azide | 90% H2O/10% D2O | 200 | 6.5 | ambient | 298 | |
3 | 2D 1H-15N HSQC | 1 mM [U-100% 13C; U-100% 15N] nsr437a, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 50 uM DSS, 0.02 % sodium azide | 90% H2O/10% D2O | 200 | 6.5 | ambient | 298 | |
4 | 2D 1H-13C HSQC aliphatic | 1 mM [U-100% 13C; U-100% 15N] nsr437a, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 50 uM DSS, 0.02 % sodium azide | 90% H2O/10% D2O | 200 | 6.5 | ambient | 298 | |
5 | 2D 1H-13C CT-HSQC aliphatic | 1 mM [U-100% 13C; U-100% 15N] nsr437a, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 50 uM DSS, 0.02 % sodium azide | 90% H2O/10% D2O | 200 | 6.5 | ambient | 298 | |
6 | 2D 1H-13C CT-HSQC aromatic | 1 mM [U-100% 13C; U-100% 15N] nsr437a, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 50 uM DSS, 0.02 % sodium azide | 90% H2O/10% D2O | 200 | 6.5 | ambient | 298 | |
7 | 3D HNCO | 1 mM [U-100% 13C; U-100% 15N] nsr437a, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 50 uM DSS, 0.02 % sodium azide | 90% H2O/10% D2O | 200 | 6.5 | ambient | 298 | |
8 | 3D HN(CA)CO | 1 mM [U-100% 13C; U-100% 15N] nsr437a, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 50 uM DSS, 0.02 % sodium azide | 90% H2O/10% D2O | 200 | 6.5 | ambient | 298 | |
9 | 3D CBCA(CO)NH | 1 mM [U-100% 13C; U-100% 15N] nsr437a, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 50 uM DSS, 0.02 % sodium azide | 90% H2O/10% D2O | 200 | 6.5 | ambient | 298 | |
10 | 3D HNCACB | 1 mM [U-100% 13C; U-100% 15N] nsr437a, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 50 uM DSS, 0.02 % sodium azide | 90% H2O/10% D2O | 200 | 6.5 | ambient | 298 | |
11 | 3D HBHA(CO)NH | 1 mM [U-100% 13C; U-100% 15N] nsr437a, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 50 uM DSS, 0.02 % sodium azide | 90% H2O/10% D2O | 200 | 6.5 | ambient | 298 | |
12 | 3D (H)CCH-TOCSY aliphatic | 1 mM [U-100% 13C; U-100% 15N] nsr437a, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 50 uM DSS, 0.02 % sodium azide | 90% H2O/10% D2O | 200 | 6.5 | ambient | 298 | |
13 | 3D (H)CCH-COSY aliphatic | 1 mM [U-100% 13C; U-100% 15N] nsr437a, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 50 uM DSS, 0.02 % sodium azide | 90% H2O/10% D2O | 200 | 6.5 | ambient | 298 | |
14 | 3D (H)CCH-COSY aromatic | 1 mM [U-100% 13C; U-100% 15N] nsr437a, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 50 uM DSS, 0.02 % sodium azide | 90% H2O/10% D2O | 200 | 6.5 | ambient | 298 | |
15 | 3D 1H-15N/13C NOESY | 1 mM [U-100% 13C; U-100% 15N] nsr437a, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 50 uM DSS, 0.02 % sodium azide | 90% H2O/10% D2O | 200 | 6.5 | ambient | 298 | |
16 | 2D 1H-15N HSQC long-range (His) | 1 mM [U-2% 13C; U-100% 15N] nsr437a, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 50 uM DSS, 0.02 % sodium azide | 90% H2O/10% D2O | 200 | 6.5 | ambient | 298 | |
17 | 2D 1H-13C CT-HSQC methyl | 1 mM [U-2% 13C; U-100% 15N] nsr437a, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 50 uM DSS, 0.02 % sodium azide | 90% H2O/10% D2O | 200 | 6.5 | ambient | 298 | |
18 | 2D 1H-15N HSQC | 1 mM [U-2% 13C; U-100% 15N] nsr437a, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 50 uM DSS, 0.02 % sodium azide | 90% H2O/10% D2O | 200 | 6.5 | ambient | 298 | |
19 | 2D 1H-15N TROSY | 1 mM [U-2% 13C; U-100% 15N] nsr437a, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 50 uM DSS, 0.02 % sodium azide | 90% H2O/10% D2O | 200 | 6.5 | ambient | 298 | |
20 | 2D 1H-15N HSQC | 0.7 mM [U-2% 13C; U-100% 15N] nsr437a, 14.4 mM MES, 144 mM sodium chloride, 3.6 mM calcium chloride, 36 uM DSS, 0.014 % sodium azide, 13.25 g/l Pf1 phage | 87% H2O/13% D2O | 150 | 6.5 | ambient | 298 | |
21 | 2D 1H-15N TROSY | 0.7 mM [U-2% 13C; U-100% 15N] nsr437a, 14.4 mM MES, 144 mM sodium chloride, 3.6 mM calcium chloride, 36 uM DSS, 0.014 % sodium azide, 13.25 g/l Pf1 phage | 87% H2O/13% D2O | 150 | 6.5 | ambient | 298 | |
22 | 2D 1H-15N HSQC | 0.7 mM [U-2% 13C; U-100% 15N] nsr437a, 16 mM MES-21, 160 mM sodium chloride, 4 mM calcium chloride-23, 40 uM DSS, 0.016 % sodium azide, 4.2 % PEG | 88% H2O/12% D2O | 160 | 6.5 | ambient | 298 | |
23 | 2D 1H-15N TROSY | 0.7 mM [U-2% 13C; U-100% 15N] nsr437a, 16 mM MES-21, 160 mM sodium chloride, 4 mM calcium chloride-23, 40 uM DSS, 0.016 % sodium azide, 4.2 % PEG | 88% H2O/12% D2O | 160 | 6.5 | ambient | 298 | |
24 | 2D CLEANEX | 1 mM [U-100% 13C; U-100% 15N] nsr437a, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 50 uM DSS, 0.02 % sodium azide | 90% H2O/10% D2O | 200 | 6.5 | ambient | 298 | |