NMR Experiment |
---|
Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
---|
1 | 2D 1H-15N HSQC | 0.7 mM [U-99% 13C; U-99% 15N] SlR105H, 10 % [U-2H] D2O, 90 % H2O, 50 uM DSS, 10 mM DTT, 20 uM zinc sulfate, 0.02 % sodium azide, 450 mM sodium chloride, 25 mM sodium phosphate | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
2 | 2D 1H-13C HSQC | 0.7 mM [U-99% 13C; U-99% 15N] SlR105H, 10 % [U-2H] D2O, 90 % H2O, 50 uM DSS, 10 mM DTT, 20 uM zinc sulfate, 0.02 % sodium azide, 450 mM sodium chloride, 25 mM sodium phosphate | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
3 | 3D HNNCO | 0.7 mM [U-99% 13C; U-99% 15N] SlR105H, 10 % [U-2H] D2O, 90 % H2O, 50 uM DSS, 10 mM DTT, 20 uM zinc sulfate, 0.02 % sodium azide, 450 mM sodium chloride, 25 mM sodium phosphate | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
4 | 3D HNCACB | 0.7 mM [U-99% 13C; U-99% 15N] SlR105H, 10 % [U-2H] D2O, 90 % H2O, 50 uM DSS, 10 mM DTT, 20 uM zinc sulfate, 0.02 % sodium azide, 450 mM sodium chloride, 25 mM sodium phosphate | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
5 | 3D CBCA(CO)NH | 0.7 mM [U-99% 13C; U-99% 15N] SlR105H, 10 % [U-2H] D2O, 90 % H2O, 50 uM DSS, 10 mM DTT, 20 uM zinc sulfate, 0.02 % sodium azide, 450 mM sodium chloride, 25 mM sodium phosphate | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
6 | 3D HBHA(CO)NH | 0.7 mM [U-99% 13C; U-99% 15N] SlR105H, 10 % [U-2H] D2O, 90 % H2O, 50 uM DSS, 10 mM DTT, 20 uM zinc sulfate, 0.02 % sodium azide, 450 mM sodium chloride, 25 mM sodium phosphate | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
7 | 3D HCCH-TOCSY | 0.7 mM [U-99% 13C; U-99% 15N] SlR105H, 10 % [U-2H] D2O, 90 % H2O, 50 uM DSS, 10 mM DTT, 20 uM zinc sulfate, 0.02 % sodium azide, 450 mM sodium chloride, 25 mM sodium phosphate | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
8 | 3D simultaneous NCaliCaro HH NOESY | 0.7 mM [U-99% 13C; U-99% 15N] SlR105H, 10 % [U-2H] D2O, 90 % H2O, 50 uM DSS, 10 mM DTT, 20 uM zinc sulfate, 0.02 % sodium azide, 450 mM sodium chloride, 25 mM sodium phosphate | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
9 | 2D 1H-13C HSQC | 0.7 mM [U-5% 13C; U-99% 15N] SlR105H, 10 % [U-2H] D2O, 90 % H2O, 50 uM DSS, 10 mM DTT, 20 uM zinc sulfate, 0.02 % sodium azide, 450 mM sodium chloride, 25 mM sodium phosphate | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |