NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 2D 1H-15N HSQC | 550 uM [U-100% 13C; U-100% 15N] PDZ2-270-1, 20 mM HEPES-2, 0.5 mM DTT-3, 0.1 mM PMSF-4, 150 mM sodium chloride-5 | 90% H2O/10% D2O | 0.150 | 7.5 | ambient | 303 | |
2 | 2D 1H-13C HSQC | 550 uM [U-100% 13C; U-100% 15N] PDZ2-270-1, 20 mM HEPES-2, 0.5 mM DTT-3, 0.1 mM PMSF-4, 150 mM sodium chloride-5 | 90% H2O/10% D2O | 0.150 | 7.5 | ambient | 303 | |
3 | 3D HNCACB | 550 uM [U-100% 13C; U-100% 15N] PDZ2-270-1, 20 mM HEPES-2, 0.5 mM DTT-3, 0.1 mM PMSF-4, 150 mM sodium chloride-5 | 90% H2O/10% D2O | 0.150 | 7.5 | ambient | 303 | |
4 | 3D HCCH-TOCSY | 550 uM [U-100% 13C; U-100% 15N] PDZ2-270-1, 20 mM HEPES-2, 0.5 mM DTT-3, 0.1 mM PMSF-4, 150 mM sodium chloride-5 | 90% H2O/10% D2O | 0.150 | 7.5 | ambient | 303 | |
5 | 3D H(CCO)NH | 550 uM [U-100% 13C; U-100% 15N] PDZ2-270-1, 20 mM HEPES-2, 0.5 mM DTT-3, 0.1 mM PMSF-4, 150 mM sodium chloride-5 | 90% H2O/10% D2O | 0.150 | 7.5 | ambient | 303 | |
6 | 3D 1H-15N NOESY | 550 uM [U-100% 13C; U-100% 15N] PDZ2-270-1, 20 mM HEPES-2, 0.5 mM DTT-3, 0.1 mM PMSF-4, 150 mM sodium chloride-5 | 90% H2O/10% D2O | 0.150 | 7.5 | ambient | 303 | |
7 | 3D 1H-13C NOESY | 550 uM [U-100% 13C; U-100% 15N] PDZ2-270-1, 20 mM HEPES-2, 0.5 mM DTT-3, 0.1 mM PMSF-4, 150 mM sodium chloride-5 | 90% H2O/10% D2O | 0.150 | 7.5 | ambient | 303 | |