NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 3D 1H-15N NOESY | 1 mM [U-99% 13C; U-99% 15N] protein, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 0.02 w/v sodium azide, 95 % H2O, 5 % [U-99% 2H] D2O | 95% H2O/5% D2O | 215 | 6.5 | ambient | 293 | |
2 | 3D 1H-13C NOESY | 1 mM [U-99% 13C; U-99% 15N] protein, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 0.02 w/v sodium azide, 95 % H2O, 5 % [U-99% 2H] D2O | 95% H2O/5% D2O | 215 | 6.5 | ambient | 293 | |
3 | 3D HNCO | 1 mM [U-99% 13C; U-99% 15N] protein, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 0.02 w/v sodium azide, 95 % H2O, 5 % [U-99% 2H] D2O | 95% H2O/5% D2O | 215 | 6.5 | ambient | 293 | |
4 | 3D HNCA | 1 mM [U-99% 13C; U-99% 15N] protein, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 0.02 w/v sodium azide, 95 % H2O, 5 % [U-99% 2H] D2O | 95% H2O/5% D2O | 215 | 6.5 | ambient | 293 | |
5 | 3D HN(CO)CA | 1 mM [U-99% 13C; U-99% 15N] protein, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 0.02 w/v sodium azide, 95 % H2O, 5 % [U-99% 2H] D2O | 95% H2O/5% D2O | 215 | 6.5 | ambient | 293 | |
6 | 3D CBCA(CO)NH | 1 mM [U-99% 13C; U-99% 15N] protein, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 0.02 w/v sodium azide, 95 % H2O, 5 % [U-99% 2H] D2O | 95% H2O/5% D2O | 215 | 6.5 | ambient | 293 | |
7 | 3D HNCACB | 1 mM [U-99% 13C; U-99% 15N] protein, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 0.02 w/v sodium azide, 95 % H2O, 5 % [U-99% 2H] D2O | 95% H2O/5% D2O | 215 | 6.5 | ambient | 293 | |
8 | 3D HBHA(CO)NH | 1 mM [U-99% 13C; U-99% 15N] protein, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 0.02 w/v sodium azide, 95 % H2O, 5 % [U-99% 2H] D2O | 95% H2O/5% D2O | 215 | 6.5 | ambient | 293 | |
9 | 3D C(CO)NH | 1 mM [U-99% 13C; U-99% 15N] protein, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 0.02 w/v sodium azide, 95 % H2O, 5 % [U-99% 2H] D2O | 95% H2O/5% D2O | 215 | 6.5 | ambient | 293 | |
10 | 3D HCCH-TOCSY | 1 mM [U-99% 13C; U-99% 15N] protein, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 0.02 w/v sodium azide, 95 % H2O, 5 % [U-99% 2H] D2O | 95% H2O/5% D2O | 215 | 6.5 | ambient | 293 | |
11 | 3D HNHA | 1 mM [U-99% 13C; U-99% 15N] protein, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 0.02 w/v sodium azide, 95 % H2O, 5 % [U-99% 2H] D2O | 95% H2O/5% D2O | 215 | 6.5 | ambient | 293 | |
12 | 2D 1H-15N HSQC | 1 mM [U-99% 13C; U-99% 15N] protein, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 0.02 w/v sodium azide, 95 % H2O, 5 % [U-99% 2H] D2O | 95% H2O/5% D2O | 215 | 6.5 | ambient | 293 | |
13 | 2D 1H-13C HSQC | 0.7 mM [5% biosynthetically directed 13C; U-99% 15N] protein, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 0.02 w/v sodium azide, 95 % H2O, 5 % [U-99% 2H] D2O | 95% H2O/5% D2O | 215 | 6.5 | ambient | 293 | |
14 | 4D 1H-13C-13C-1H HMQC-NOESY-HMQC | 0.7 mM [U-99% 13C; U-99% 15N] protein, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 0.02 w/v sodium azide, 100 % [U-99% 2H] D2O-20 | 100% D2O | 215 | 6.5 | ambient | 293 | |