2KHJ
NMR structure of the domain 6 of the E. coli ribosomal protein S1
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 15N-NOESY-HSQC 80ms | 0.5-0.7mM [U-100% 15N] Domain 6 of the E. coli ribosomal protein S1-1 | 95% H2O, 10% D2O | 200 | 6.8 | ambient | 303 | |
| 2 | 13Caliphatic-NOESY-HSQC 80 ms | 0.5-0.7mM [U-100% 13C; U-100% 15N] Domain 6 of the E. coli ribosomal protein S1-2 | 100% D2O | 200 | 6.8 | ambient | 303 | |
| 3 | 2D-NOESY 100 ms | 0.5-0.7mM [U-100% 15N] Domain 6 of the E. coli ribosomal protein S1-3 | 100% D2O | 200 | 6.8 | ambient | 303 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | DRX | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| torsion angle dynamics, simulated annealing | INCA | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | 12 structures for lowest energy |
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 12 |
| Representative Model | 1 (n/a) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | structure solution | INCA | Gilquin B. | |
| 2 | refinement | INCA | Gilquin B. | |
