2KD8
Solution structure of the stem-loop IIId of GBV-B IRES
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 2D NOESY | 1-1.6 mM RNA | 90% H2O/10% D2O | 6.8 | ambient | 298 | ||
| 2 | 2D DQF-COSY | 1-1.6 mM RNA | 90% H2O/10% D2O | 6.8 | ambient | 298 | ||
| 3 | 2D 1H-13C HSQC | 1-1.6 mM RNA | 90% H2O/10% D2O | 6.8 | ambient | 298 | ||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | INOVA | 750 |
| 2 | Varian | INOVA | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| distance geometry, molecular dynamics, matrix relaxation | VnmrJ | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the lowest energy |
| Conformers Calculated Total Number | 10 |
| Conformers Submitted Total Number | 12 |
| Representative Model | 1 (closest to the average) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | chemical shift assignment | VnmrJ | Varian | |
| 2 | refinement | X-PLOR | 3.1 | Brunger |
