NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 2D 1H-15N HSQC | 0.45 mM [U-99% 13C; U-99% 15N] CtR107-1, 5 % [U-2H] D2O-2, 95 % H2O-3, 50 uM DSS-4, 10 mM DTT-5, 0.02 % sodium azide-6, 5 mM calcium chloride-7, 100 mM sodium chloride-8, 20 mM MES-9 | 90% H2O/10% D2O | 235 | 6.5 | ambient | 298 | |
2 | 2D 1H-13C HSQC | 0.45 mM [U-99% 13C; U-99% 15N] CtR107-1, 5 % [U-2H] D2O-2, 95 % H2O-3, 50 uM DSS-4, 10 mM DTT-5, 0.02 % sodium azide-6, 5 mM calcium chloride-7, 100 mM sodium chloride-8, 20 mM MES-9 | 90% H2O/10% D2O | 235 | 6.5 | ambient | 298 | |
3 | 3D HNCO | 0.45 mM [U-99% 13C; U-99% 15N] CtR107-1, 5 % [U-2H] D2O-2, 95 % H2O-3, 50 uM DSS-4, 10 mM DTT-5, 0.02 % sodium azide-6, 5 mM calcium chloride-7, 100 mM sodium chloride-8, 20 mM MES-9 | 90% H2O/10% D2O | 235 | 6.5 | ambient | 298 | |
4 | 3D HNCACB | 0.45 mM [U-99% 13C; U-99% 15N] CtR107-1, 5 % [U-2H] D2O-2, 95 % H2O-3, 50 uM DSS-4, 10 mM DTT-5, 0.02 % sodium azide-6, 5 mM calcium chloride-7, 100 mM sodium chloride-8, 20 mM MES-9 | 90% H2O/10% D2O | 235 | 6.5 | ambient | 298 | |
5 | 3D CBCA(CO)NH | 0.45 mM [U-99% 13C; U-99% 15N] CtR107-1, 5 % [U-2H] D2O-2, 95 % H2O-3, 50 uM DSS-4, 10 mM DTT-5, 0.02 % sodium azide-6, 5 mM calcium chloride-7, 100 mM sodium chloride-8, 20 mM MES-9 | 90% H2O/10% D2O | 235 | 6.5 | ambient | 298 | |
6 | 3D HBHA(CO)NH | 0.45 mM [U-99% 13C; U-99% 15N] CtR107-1, 5 % [U-2H] D2O-2, 95 % H2O-3, 50 uM DSS-4, 10 mM DTT-5, 0.02 % sodium azide-6, 5 mM calcium chloride-7, 100 mM sodium chloride-8, 20 mM MES-9 | 90% H2O/10% D2O | 235 | 6.5 | ambient | 298 | |
7 | 3D HCCH-COSY | 0.45 mM [U-99% 13C; U-99% 15N] CtR107-1, 5 % [U-2H] D2O-2, 95 % H2O-3, 50 uM DSS-4, 10 mM DTT-5, 0.02 % sodium azide-6, 5 mM calcium chloride-7, 100 mM sodium chloride-8, 20 mM MES-9 | 90% H2O/10% D2O | 235 | 6.5 | ambient | 298 | |
8 | 3D simultaneous NCaliCaro HH NOESY | 0.45 mM [U-99% 13C; U-99% 15N] CtR107-1, 5 % [U-2H] D2O-2, 95 % H2O-3, 50 uM DSS-4, 10 mM DTT-5, 0.02 % sodium azide-6, 5 mM calcium chloride-7, 100 mM sodium chloride-8, 20 mM MES-9 | 90% H2O/10% D2O | 235 | 6.5 | ambient | 298 | |
9 | 2D 1H-13C HSQC | 0.45 mM [U-5% 13C; U-99% 15N] CtR107-10, 5 % [U-2H] D2O-11, 95 % H2O-12, 50 uM DSS-13, 10 mM DTT-14, 0.02 % sodium azide-15, 5 mM calcium chloride-16, 100 mM sodium chloride-17, 20 mM MES-18 | 90% H2O/10% D2O | 235 | 6.5 | ambient | 298 | |
10 | 2D 1H-15N HSQC | 0.45 mM [U-5% 13C; U-99% 15N] CtR107-10, 5 % [U-2H] D2O-11, 95 % H2O-12, 50 uM DSS-13, 10 mM DTT-14, 0.02 % sodium azide-15, 5 mM calcium chloride-16, 100 mM sodium chloride-17, 20 mM MES-18 | 90% H2O/10% D2O | 235 | 6.5 | ambient | 298 | |