2K9H

The hantavirus glycoprotein G1 tail contains a dual CCHC-type classical zinc fingers

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	2D 1H-15N HSQC	10 mM sodium phosphate, 10 mM sodium chloride, 1 mM DTT, 0.1 mM ZINC ION, 1 mM [U-100% 15N] Protein	90% H2O/10% D2O	70	7.0	ambient	298
2	3D HNCA	10 mM sodium phosphate, 10 mM sodium chloride, 1 mM DTT, 0.1 mM ZINC ION, 1 mM [U-100% 13C; U-100% 15N] Protein	90% H2O/10% D2O	70	7.0	ambient	298
3	3D HNCO	10 mM sodium phosphate, 10 mM sodium chloride, 1 mM DTT, 0.1 mM ZINC ION, 1 mM [U-100% 13C; U-100% 15N] Protein	90% H2O/10% D2O	70	7.0	ambient	298
4	3D CBCA(CO)NH	10 mM sodium phosphate, 10 mM sodium chloride, 1 mM DTT, 0.1 mM ZINC ION, 1 mM [U-100% 13C; U-100% 15N] Protein	90% H2O/10% D2O	70	7.0	ambient	298
5	3D HNCACB	10 mM sodium phosphate, 10 mM sodium chloride, 1 mM DTT, 0.1 mM ZINC ION, 1 mM [U-100% 13C; U-100% 15N] Protein	90% H2O/10% D2O	70	7.0	ambient	298
6	3D HBHA(CO)NH	10 mM sodium phosphate, 10 mM sodium chloride, 1 mM DTT, 0.1 mM ZINC ION, 1 mM [U-100% 13C; U-100% 15N] Protein	90% H2O/10% D2O	70	7.0	ambient	298
7	3D 1H-15N NOESY	10 mM sodium phosphate, 10 mM sodium chloride, 1 mM DTT, 0.1 mM ZINC ION, 1 mM [U-100% 15N] Protein	90% H2O/10% D2O	70	7.0	ambient	298
8	3D 1H-13C NOESY	10 mM sodium phosphate, 10 mM sodium chloride, 1 mM DTT, 0.1 mM ZINC ION, 1 mM [U-100% 13C; U-100% 15N] Protein	100% D2O	70	7.0	ambient	298
9	2D 1H-13C HSQC	10 mM sodium phosphate, 10 mM sodium chloride, 1 mM DTT, 0.1 mM ZINC ION, 1 mM [U-100% 13C; U-100% 15N] Protein	100% D2O	70	7.0	ambient	298

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	AVANCE	800

NMR Refinement
Method	Details	Software
simulated annealing		Amber

NMR Ensemble Information
Conformer Selection Criteria	structures with the lowest energy
Conformers Calculated Total Number	40
Conformers Submitted Total Number	20
Representative Model	1 (lowest energy)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	refinement	Amber	Amber 7	Case, D.A. et al.
2	processing	NMRPipe		Delaglio, F. et al.
3	collection	TopSpin		Bruker Biospin
4	chemical shift assignment	NMRView		Johnson, B. et al.
5	data analysis	NMRView		Johnson, B. et al.
6	peak picking	NMRView		Johnson, B. et al.
7	processing	NMRDraw		Delaglio, F. et al.
8	structure solution	CYANA		Guntert, P. et al.

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.