2K93
Structural modification of acyl carrier protein by butyryl group
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 3D 1H-13C NOESY | 1.5 mM [U-100% 13C; U-100% 15N] Holo ACP, 40 mM potassium phosphate | 90% H2O/10% D2O | 6.5 | ||||
| 2 | 3D 1H-15N NOESY | 1.5 mM [U-100% 13C; U-100% 15N] Holo ACP, 40 mM potassium phosphate | 90% H2O/10% D2O | 6.5 | ||||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | INOVA | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| torsion angle dynamics | DYANA | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | target function |
| Conformers Calculated Total Number | 200 |
| Conformers Submitted Total Number | 20 |
| Representative Model | 1 (fewest violations) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | structure solution | DYANA | Guntert, Braun and Wuthrich | |
| 2 | refinement | DYANA | Guntert, Braun and Wuthrich | |
