| NMR Experiment |
|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
|---|
| 1 | 2D 1H-1H NOESY | 0.3 mM RNA (5'-R(*GP*CP*GP*GP*UP*GP*UP*AP*AP*AP*AP*G)-3'), 0.3 mM T-RNA (5'-R(*CP*UP*(SUR)P*UP*UP*AP*AP*(PSU)P*CP*UP*GP*C)-3'), 10 mM sodium phosphate, 100 mM sodium chloride | 90% H2O/10% D2O | 0.1 | 6.5 | ambient | 276 | |
| 2 | 2D 1H-1H NOESY | 0.3 mM RNA (5'-R(*GP*CP*GP*GP*UP*GP*UP*AP*AP*AP*AP*G)-3'), 0.3 mM T-RNA (5'-R(*CP*UP*(SUR)P*UP*UP*AP*AP*(PSU)P*CP*UP*GP*C)-3'), 10 mM sodium phosphate, 100 mM sodium chloride | 90% H2O/10% D2O | 0.1 | 6.5 | ambient | 293 | |
| 3 | 2D 1H-13C HSQC | 0.3 mM RNA (5'-R(*GP*CP*GP*GP*UP*GP*UP*AP*AP*AP*AP*G)-3'), 0.3 mM T-RNA (5'-R(*CP*UP*(SUR)P*UP*UP*AP*AP*(PSU)P*CP*UP*GP*C)-3'), 10 mM sodium phosphate, 100 mM sodium chloride | 100% D2O | 0.1 | 6.5 | ambient | 293 | |
| 4 | 2D DQF-COSY | 0.3 mM RNA (5'-R(*GP*CP*GP*GP*UP*GP*UP*AP*AP*AP*AP*G)-3'), 0.3 mM T-RNA (5'-R(*CP*UP*(SUR)P*UP*UP*AP*AP*(PSU)P*CP*UP*GP*C)-3'), 10 mM sodium phosphate, 100 mM sodium chloride | 100% D2O | 0.1 | 6.5 | ambient | 293 | |
| 5 | 2D 1H-1H NOESY | 0.3 mM RNA (5'-R(*GP*CP*GP*GP*UP*GP*UP*AP*AP*AP*AP*G)-3'), 0.3 mM T-RNA (5'-R(*CP*UP*(SUR)P*UP*UP*AP*AP*(PSU)P*CP*UP*GP*C)-3'), 10 mM sodium phosphate, 100 mM sodium chloride | 100% D2O | 0.1 | 6.5 | ambient | 293 | |
| 6 | 2D 1H-15N HSQC | 0.3 mM RNA (5'-R(*GP*CP*GP*GP*UP*GP*UP*AP*AP*AP*AP*G)-3'), 0.3 mM T-RNA (5'-R(*CP*UP*(SUR)P*UP*UP*AP*AP*(PSU)P*CP*UP*GP*C)-3'), 10 mM sodium phosphate, 100 mM sodium chloride | 90% H2O/10% D2O | 0.1 | 6.5 | ambient | 276 | |
