2JZ6

Solution structure of 50S ribosomal protein L28 from Thermotoga maritima. Northeast Structural Genomics Consortium target VR97

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	4,3D, GFT HNNCABCA	1.0 mM protein	90% H2O/10% D2O	0.3	7.0	ambient	298
2	4,3D, GFT CABCACONNH	1.0 mM protein	90% H2O/10% D2O	0.3	7.0	ambient	298
3	4,3D, GFT HCCH COSY	1.0 mM protein	90% H2O/10% D2O	0.3	7.0	ambient	298
4	3D, 15N-13C RESOLVEDSIMULTANEOUS NOESY	1.0 mM protein	90% H2O/10% D2O	0.3	7.0	ambient	298
5	3D HNCO	1.0 mM protein	90% H2O/10% D2O	0.3	7.0	ambient	298
6	2D 1H-15N HSQC	1.0 mM protein	90% H2O/10% D2O	0.3	7.0	ambient	298
7	2D 1H-13C HSQC	1.0 mM protein	90% H2O/10% D2O	0.3	7.0	ambient	298

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Varian	INOVA	750
2	Varian	INOVA	600

NMR Refinement
Method	Details	Software
simulated annealing		CNS

NMR Ensemble Information
Conformer Selection Criteria	target function
Conformers Calculated Total Number	100
Conformers Submitted Total Number	20
Representative Model	1 (lowest energy)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	refinement	CNS		Brunger, Adams, Clore, Gros, Nilges and Read
2	chemical shift assignment	AutoAssign		Zimmerman, Moseley, Kulikowski and Montelione
3	peak picking	XEASY		Bartels et al.
4	processing	NMRPipe		Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
5	structure solution	AutoStructure		Huang, Tejero, Powers and Montelione
6	structure solution	CYANA		Guntert, Mumenthaler and Wuthrich

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.