NMR Experiment |
---|
Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
---|
1 | 2D 1H-15N HSQC | 0.5 mM [U-13C; U-15N] HP0495, 10 mM TRIS, 300 mM sodium chloride, 1 mM benzamidine, 10 mM DTT, 0.01 % sodium azide | 95% H2O/5% D2O | 300 | 7 | ambient | 298 | |
2 | 2D 1H-13C HSQC | 0.5 mM [U-13C; U-15N] HP0495, 10 mM TRIS, 300 mM sodium chloride, 1 mM benzamidine, 10 mM DTT, 0.01 % sodium azide | 95% H2O/5% D2O | 300 | 7 | ambient | 298 | |
3 | 3D CBCA(CO)NH | 0.5 mM [U-13C; U-15N] HP0495, 10 mM TRIS, 300 mM sodium chloride, 1 mM benzamidine, 10 mM DTT, 0.01 % sodium azide | 95% H2O/5% D2O | 300 | 7 | ambient | 298 | |
4 | 3D HNCO sparse | 0.5 mM [U-13C; U-15N] HP0495, 10 mM TRIS, 300 mM sodium chloride, 1 mM benzamidine, 10 mM DTT, 0.01 % sodium azide | 95% H2O/5% D2O | 300 | 7 | ambient | 298 | |
5 | 3D HNCA sparse | 0.5 mM [U-13C; U-15N] HP0495, 10 mM TRIS, 300 mM sodium chloride, 1 mM benzamidine, 10 mM DTT, 0.01 % sodium azide | 95% H2O/5% D2O | 300 | 7 | ambient | 298 | |
6 | 3D H(CCCO)NH-TOCSY sparse | 0.5 mM [U-13C; U-15N] HP0495, 10 mM TRIS, 300 mM sodium chloride, 1 mM benzamidine, 10 mM DTT, 0.01 % sodium azide | 95% H2O/5% D2O | 300 | 7 | ambient | 298 | |
7 | 3D (H)C(CCO)NH-TOCSY | 0.5 mM [U-13C; U-15N] HP0495, 10 mM TRIS, 300 mM sodium chloride, 1 mM benzamidine, 10 mM DTT, 0.01 % sodium azide | 95% H2O/5% D2O | 300 | 7 | ambient | 298 | |
8 | 4D HBHACBCA(CO)NH PR | 0.5 mM [U-13C; U-15N] HP0495, 10 mM TRIS, 300 mM sodium chloride, 1 mM benzamidine, 10 mM DTT, 0.01 % sodium azide | 95% H2O/5% D2O | 300 | 7 | ambient | 298 | |
9 | 4D HC(CCO)NH-TOCSY PR | 0.5 mM [U-13C; U-15N] HP0495, 10 mM TRIS, 300 mM sodium chloride, 1 mM benzamidine, 10 mM DTT, 0.01 % sodium azide | 95% H2O/5% D2O | 300 | 7 | ambient | 298 | |
10 | 3D 1H-15N NOESY sparse | 0.5 mM [U-13C; U-15N] HP0495, 10 mM TRIS, 300 mM sodium chloride, 1 mM benzamidine, 10 mM DTT, 0.01 % sodium azide | 95% H2O/5% D2O | 300 | 7 | ambient | 298 | |
11 | 3D 1H-13C NOESY aliphatic | 0.5 mM [U-13C; U-15N] HP0495, 10 mM TRIS, 300 mM sodium chloride, 1 mM benzamidine, 10 mM DTT, 0.01 % sodium azide | 95% H2O/5% D2O | 300 | 7 | ambient | 298 | |
12 | 3D H(C)CH-TOCSY sparse | 0.5 mM [U-13C; U-15N] HP0495, 10 mM TRIS, 300 mM sodium chloride, 1 mM benzamidine, 10 mM DTT, 0.01 % sodium azide | 100% D2O | 300 | 7 | ambient | 298 | |
13 | 3D (H)CCH-TOCSY sparse | 0.5 mM [U-13C; U-15N] HP0495, 10 mM TRIS, 300 mM sodium chloride, 1 mM benzamidine, 10 mM DTT, 0.01 % sodium azide | 100% D2O | 300 | 7 | ambient | 298 | |
14 | 3D 1H-13C NOESY aromatic | 0.5 mM [U-13C; U-15N] HP0495, 10 mM TRIS, 300 mM sodium chloride, 1 mM benzamidine, 10 mM DTT, 0.01 % sodium azide | 95% H2O/5% D2O | 300 | 7 | ambient | 298 | |
15 | 3D 1H-13C NOESY sparse | 0.5 mM [U-13C; U-15N] HP0495, 10 mM TRIS, 300 mM sodium chloride, 1 mM benzamidine, 10 mM DTT, 0.01 % sodium azide | 100% D2O | 300 | 7 | ambient | 298 | |
16 | 3D H(C)CH-TOCSY aromatic, sparse | 0.5 mM [U-13C; U-15N] HP0495, 10 mM TRIS, 300 mM sodium chloride, 1 mM benzamidine, 10 mM DTT, 0.01 % sodium azide | 100% D2O | 300 | 7 | ambient | 298 | |
17 | 3D (H)CCH-TOCSY aromatic | 0.5 mM [U-13C; U-15N] HP0495, 10 mM TRIS, 300 mM sodium chloride, 1 mM benzamidine, 10 mM DTT, 0.01 % sodium azide | 100% D2O | 300 | 7 | ambient | 298 | |
18 | 2D CT 1H-13C HSQC | 0.5 mM [U-13C; U-15N] HP0495, 10 mM TRIS, 300 mM sodium chloride, 1 mM benzamidine, 10 mM DTT, 0.01 % sodium azide | 100% D2O | 300 | 7 | ambient | 298 | |
19 | 2D CT 1H-13C HSQC aromatic | 0.5 mM [U-13C; U-15N] HP0495, 10 mM TRIS, 300 mM sodium chloride, 1 mM benzamidine, 10 mM DTT, 0.01 % sodium azide | 100% D2O | 300 | 7 | ambient | 298 | |