2J10
p53 tetramerization domain mutant T329F Q331K
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | NOESY | 5% D2O/95% WATER | 7.2 | 1.0 atm | 300.0 | |||
| 2 | TOCSY | 5% D2O/95% WATER | 7.2 | 1.0 atm | 300.0 | |||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | AMX | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| CNS | CNS | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | TOTAL ENERGY |
| Conformers Calculated Total Number | 30 |
| Conformers Submitted Total Number | 30 |
| Representative Model | 5 (n/a) |
| Additional NMR Experimental Information | |
|---|---|
| Details | NONE |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | CNS | BRUNGER,ADAMS,CLORE,DELANO,GROS, GROSSE- KUNSTLEVE,JIANG,KUSZEWSKI,NILGES, PANNU,READ, RICE,SIMONSON,WARREN | |
| 2 | structure solution | SPARKY | ||
