2IY3
Structure of the E. Coli Signal Regognition Particle
ELECTRON MICROSCOPY
Sample |
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SRP BOUND TO RIBOSOME |
Specimen Preparation | |
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Sample Aggregation State | PARTICLE |
Vitrification Instrument | HOMEMADE PLUNGER |
Cryogen Name | ETHANE |
Sample Vitrification Details | PLUNGED INTO ETHANE |
3D Reconstruction | |
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Reconstruction Method | SINGLE PARTICLE |
Number of Particles | 24382 |
Reported Resolution (Å) | 16 |
Resolution Method | |
Other Details | THE COORDINATES BELOW ARE MADE UP FROM THE FOLLOWING COMPONENTS: FFH NG DOMAIN (RESIDUE 1-296), ORGANISM SCIENTIFIC: THERMUS AQUATICUS (PDB ENTRY 1JPN ... |
Refinement Type | |
Symmetry Type | POINT |
Point Symmetry | C1 |
Map-Model Fitting and Refinement | |||||
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Id | 1 (1JPN, 1QZW) | ||||
Refinement Space | REAL | ||||
Refinement Protocol | OTHER | ||||
Refinement Target | Cross-correlation coefficient | ||||
Overall B Value | |||||
Fitting Procedure | |||||
Details | METHOD--LOCAL CORRELATION REFINEMENT PROTOCOL--X-RAY |
Data Acquisition | |||||||||
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Detector Type | KODAK SO-163 FILM | ||||||||
Electron Dose (electrons/Å**2) | 10 |
Imaging Experiment | 1 |
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Date of Experiment | 2005-06-02 |
Temperature (Kelvin) | 77 |
Microscope Model | FEI TECNAI 20 |
Minimum Defocus (nm) | 1000 |
Maximum Defocus (nm) | 4500 |
Minimum Tilt Angle (degrees) | |
Maximum Tilt Angle (degrees) | |
Nominal CS | 2 |
Imaging Mode | BRIGHT FIELD |
Specimen Holder Model | |
Nominal Magnification | 50000 |
Calibrated Magnification | 51000 |
Source | FIELD EMISSION GUN |
Acceleration Voltage (kV) | 200 |
Imaging Details |
EM Software | ||
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Task | Software Package | Version |
MODEL FITTING | NOMAD | |
RECONSTRUCTION | SPIDER |
Image Processing | ||||
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CTF Correction Type | CTF Correction Details | Number of Particles Selected | Particle Selection Details | |
PHASE FLIPPING |