2IY3

Structure of the E. Coli Signal Regognition Particle


ELECTRON MICROSCOPY
Sample
SRP BOUND TO RIBOSOME
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentHOMEMADE PLUNGER
Cryogen NameETHANE
Sample Vitrification DetailsPLUNGED INTO ETHANE
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles24382
Reported Resolution (Å)16
Resolution Method
Other DetailsTHE COORDINATES BELOW ARE MADE UP FROM THE FOLLOWING COMPONENTS: FFH NG DOMAIN (RESIDUE 1-296), ORGANISM SCIENTIFIC: THERMUS AQUATICUS (PDB ENTRY 1JPN ...THE COORDINATES BELOW ARE MADE UP FROM THE FOLLOWING COMPONENTS: FFH NG DOMAIN (RESIDUE 1-296), ORGANISM SCIENTIFIC: THERMUS AQUATICUS (PDB ENTRY 1JPN CHAIN A). FFH M DOMAIN (RESIDUE 297-432), ORANISM SCIENTIFIC: SULFOLOBUS SOLFATARICUS (PDB ENTRY 1QZW CHAIN A). 4.5S RNA (RESIDUE 33- 73), ORGANISM SCIENTIFIC: SULFOLOBUS SOLFATARICUS (PDB ENTRY 1QZW, CHAIN B). 4.5S RNA (RESIDUE 1-32 AND 74-110), ORGANISM SCIENTIFIC: ESCHERICHIA COLI (PDB: SRPDB, ROSENBLAD ET AL, NUCLEIC ACID RES 21, 363-364,12:2003.
Refinement Type
Symmetry TypePOINT
Point SymmetryC1
Map-Model Fitting and Refinement
Id1 (1JPN, 1QZW)
Refinement SpaceREAL
Refinement ProtocolOTHER
Refinement TargetCross-correlation coefficient
Overall B Value
Fitting Procedure
DetailsMETHOD--LOCAL CORRELATION REFINEMENT PROTOCOL--X-RAY
Data Acquisition
Detector TypeKODAK SO-163 FILM
Electron Dose (electrons/Å**2)10
Imaging Experiment1
Date of Experiment2005-06-02
Temperature (Kelvin)77
Microscope ModelFEI TECNAI 20
Minimum Defocus (nm)1000
Maximum Defocus (nm)4500
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS2
Imaging ModeBRIGHT FIELD
Specimen Holder Model
Nominal Magnification50000
Calibrated Magnification51000
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)200
Imaging Details
EM Software
TaskSoftware PackageVersion
MODEL FITTINGNOMAD
RECONSTRUCTIONSPIDER
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
PHASE FLIPPING