2HGH

Transcription Factor IIIA zinc fingers 4-6 bound to 5S rRNA 55mer (NMR structure)

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	2D NOESY	U-15N, 90% H2O, 10% D2O	90% H2O/10% D2O	10 mM	6.5	1 atm	291
2	3D_15N-separated_NOESY	U-15N, 90% H2O, 10% D2O	90% H2O/10% D2O	10 mM	6.5	1 atm	291
3	3D_13C-separated_NOESY	U-13C;U-15N	D2O	10 mM	6.5	1 atm	291
4	3D_15N-separated_NOESY	U-2H;U-13C;U-15N, 90% H2O, 10% D2O	90% H2O/10% D2O	10 mM	6.5	1 atm	291

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	AVANCE	900
2	Bruker	DRX	800
3	Bruker	DMX	750
4	Bruker	DRX	600
5	Bruker	AMX	500

NMR Refinement
Method	Details	Software
torsion angle dynamics, molecular dynamics, generalized Born solvation model		NMRPipe

NMR Ensemble Information
Conformer Selection Criteria	structures with the lowest energy
Conformers Calculated Total Number	200
Conformers Submitted Total Number	20
Representative Model	1 (lowest energy)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	processing	NMRPipe	2.3	Delaglio
2	data analysis	NMRView	5.4	Johnson
3	structure solution	DYANA	1.5	Guntert
4	refinement	Amber	8	Case

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.