2HDC
STRUCTURE OF TRANSCRIPTION FACTOR GENESIS/DNA COMPLEX
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | NOESY | 90% WATER/10% D2O | 6.5 | 303 | ||||
| 2 | HNCA | 90% WATER/10% D2O | 6.5 | 303 | ||||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | DRX | 600 |
| 2 | Bruker | DRX | 750 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| distance geometry | DYANA | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | LEAST RESTRAINT VIOLATION |
| Conformers Calculated Total Number | 200 |
| Conformers Submitted Total Number | 20 |
| Representative Model | 1 (n/a) |
| Additional NMR Experimental Information | |
|---|---|
| Details | THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C, 15N (AND 2H)-LABELED SAMPLE |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | DYANA | 1.5 | GUNTERT, PETER, ET AL |
| 2 | structure solution | DYANA | ||
