2HC5

Solution NMR Structure of Protein yvyC from Bacillus subtilis. Northeast Structural Genomics Consortium Target SR482.


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D 15N-, 13Cali-, 13Caro-resolved NOESY1.1 mM sr482 protein 10mM Tris buffer, 100mM NaCl, 0.02% NaN3, 95% H2O / 5% D2095% H2O/5% D2O100 mM6.51 atm298
2(4,3)D GFT CABCA(CO)NHN1.1 mM sr482 protein 10mM Tris buffer, 100mM NaCl, 0.02% NaN3, 95% H2O / 5% D2095% H2O/5% D2O100 mM6.51 atm298
3(4,3)D GFT HNNCABCA1.1 mM sr482 protein 10mM Tris buffer, 100mM NaCl, 0.02% NaN3, 95% H2O / 5% D2095% H2O/5% D2O100 mM6.51 atm298
4(4,3)D GFT HABCAB(CO)NHN1.1 mM sr482 protein 10mM Tris buffer, 100mM NaCl, 0.02% NaN3, 95% H2O / 5% D2095% H2O/5% D2O100 mM6.51 atm298
5(4,3)D GFT HCCH-COSY (ali)1.1 mM sr482 protein 10mM Tris buffer, 100mM NaCl, 0.02% NaN3, 95% H2O / 5% D2095% H2O/5% D2O100 mM6.51 atm298
6(4,3)D GFT HCCH-COSY (aro)1.1 mM sr482 protein 10mM Tris buffer, 100mM NaCl, 0.02% NaN3, 95% H2O / 5% D2095% H2O/5% D2O100 mM6.51 atm298
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1VarianINOVA750
NMR Refinement
MethodDetailsSoftware
torsion angle dynamicsExplicit water bath refinementVNMR
NMR Ensemble Information
Conformer Selection Criteriastructures with the least restraint violations
Conformers Calculated Total Number100
Conformers Submitted Total Number20
Representative Model1 (lowest energy)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1collectionVNMR6.1CVarian, Inc.
2data analysisXEASY1.3.13Bartels, Ch.
3data analysisCARA1.5.3Keller, R.
4processingPROSA6.0Guentert, P.
5structure solutionCYANA2.1Guentert, P.
6refinementCYANA2.1Guentert, P.