2GRG

Solution NMR Structure of Protein YNR034W-A from Saccharomyces cerevisiae. Northeast Structural Genomics Consortium Target YT727; Ontario Center for Structural Proteomics Target yst6499.

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	3D_15N-separated_NOESY	1mM yst6499, U-15N,13C; 250 mM NaCl, 10 mM Tris; 1 mM Benzamidine, 1 x inhibitor cocktail, 0.01% NaN3; 95% H2O, 5% D2O	95% H2O/5% D2O	250 mM NaCl, 10 mM Tris	7.1	ambient	298
2	3D_13C-separated_NOESY	1mM yst6499, U-15N,13C; 250 mM NaCl, 10 mM Tris; 1 mM Benzamidine, 1 x inhibitor cocktail, 0.01% NaN3; 95% H2O, 5% D2O	95% H2O/5% D2O	250 mM NaCl, 10 mM Tris	7.1	ambient	298

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Varian	INOVA	600
2	Varian	INOVA	750

NMR Refinement
Method	Details	Software
torsion angle dynamics and molecular dynamics	2890 NOE-derived distance restraints and 110 torsion angle restraints	NMRPipe

NMR Ensemble Information
Conformer Selection Criteria	structures with the lowest energy
Conformers Calculated Total Number	100
Conformers Submitted Total Number	20
Representative Model	1 (lowest energy)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	processing	NMRPipe	2.3	Delaglio, F.
2	data analysis	Sparky	3.95	Goddard, T.D. and Kneller, D.G.
3	structure solution	CYANA	2.1	Gunter, P.
4	refinement	CNS	1.0	Brunger, A.T. et al

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.