2GJY

NMR Solution Structure of Tensin1 PTB Domain


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D_15N-separated_NOESY1mM PTB domain U-15N; 50mM phosphate buffer; 150mM NaCl; 90%H2O; 10%D2O50mM phosphate buffer; 150mM NaCl; 90% H2O; 10%D2O50mM phosphate and 150mM NaCl7AMBIENT298
22D NOESY1mM PTB domain U-15N; 50mM phosphate buffer; 150mM NaCl; 100%D2O50mM phosphate buffer; 150mM NaCl; 100%D2O50mM phosphate and 150mM NaCl7AMBIENT298
33D_13C-separated_NOESY1mM PTB domain U-15N, U-13C; 50mM phosphate buffer; 150mM NaCl; 90%H2O, 10%D2O50mM phosphate buffer; 150mM NaCl; 90%H2O; 10%D2O50mM phosphate and 150mM NaCl7AMBIENT298
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerAVANCE600
NMR Refinement
MethodDetailsSoftware
torsion angle dynamics energy minimizationDYANA
NMR Ensemble Information
Conformer Selection Criteriastructures with the least restraint violations
Conformers Calculated Total Number100
Conformers Submitted Total Number20
Representative Model1 (closest to the average)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1structure solutionDYANA1.5Gunthert
2refinementGROMOS96Van Gunsteren
3processingXwinNMR3.5Bruker
4data analysisXEASY1.3.13Bartels