2GFU
NMR solution structure of the PWWP domain of Mismatch repair protein hMSH6
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 3D_13C-separated_NOESY | 1mM protein concentration, 225mM NaCl, 50mM NaPhosphate pH 6.9, 1mM TSP 10% D2O, Protease inhibitors (sigma) | 10% D2O | 225mM NaCl | 6.9 | 1 atm | 300 | |
| 2 | 3D_15N-separated_NOESY | 1mM protein concentration, 225mM NaCl, 50mM NaPhosphate pH 6.9, 1mM TSP 10% D2O, Protease inhibitors (sigma) | 10% D2O | 225mM NaCl | 6.9 | 1 atm | 300 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | DRX | 500 |
| 2 | Bruker | DRX | 600 |
| 3 | Varian | INOVA | 800 |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the lowest energy |
| Conformers Calculated Total Number | 1000 |
| Conformers Submitted Total Number | 20 |
| Representative Model | 1 (closest to the average) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | data analysis | Felix | 2000 | accelrys |
| 2 | processing | NMRPipe | 1.0 | Delaglio |
| 3 | structure solution | INCA | 1.0 | P. Savarin, B. Gilquin |
| 4 | refinement | INCA | 1.0 | P. Savarin, B. Gilquin |
