2G31
Human Nogo-A functional domain: nogo60
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 3D_15N-separated_NOESY | 90% H2O, 10% D2O | 90% H2O/10% D2O | 4.0 | ambient | 278 | ||
| 2 | 2D NOESY | 90% H2O, 10% D2O | 90% H2O/10% D2O | 4.0 | ambient | 278 | ||
| 3 | 2D TOCSY | 90% H2O, 10% D2O | 90% H2O/10% D2O | 4.0 | ambient | 278 | ||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | INOVA | 800 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| distance geometry | DYANA | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | target function |
| Conformers Calculated Total Number | 200 |
| Conformers Submitted Total Number | 20 |
| Representative Model | 1 (closest to the average) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | DYANA | CYANA 1.0.6 | Peter Guntert |
