2F3I

Solution Structure of a Subunit of RNA Polymerase II


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D_13C-separated_NOESY0.5mM RNA Polymerase II Subunit U-15N,13C20mM potassium phosphate buffer, 10mM DTT; 90% H2O, 10% D2O50mM6.3ambient303
23D_15N-separated_NOESY0.5mM RNA Polymerase II Subunit U-15N,13C20mM potassium phosphate buffer, 10mM DTT; 90% H2O, 10% D2O50mM6.3ambient303
32D_15N-edited_HSQC0.5mM RNA Polymerase II Subunit U-15N,13C20mM potassium phosphate buffer, 10mM DTT; 90% H2O, 10% D2O50mM6.3ambient303
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerAVANCE800
2BrukerAVANCE500
NMR Refinement
MethodDetailsSoftware
simulated annealing molecular dynamicsXwinNMR
NMR Ensemble Information
Conformer Selection Criteriastructures with the lowest energy
Conformers Calculated Total Number100
Conformers Submitted Total Number21
Representative Model1 (minimized average structure)
Additional NMR Experimental Information
DetailsThis structure was determined using standard 3D heteronuclear techniques.
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1collectionXwinNMR3.5Bruker
2processingNMRPipe2.1Frank Delaglio
3data analysisNMRView5Bruce Johnson
4structure solutionCYANA1.0.6Peter Gunter
5refinementAmber7.0David Case