2EYV

SH2 domain of CT10-Regulated Kinase


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D_15N-SEPARATED_NOESY1.0MM SH2 U-15N, 13C PHOSPHATE BUFFER NA; 200MM NACL;90%H2O, 10%D2O250mM6.8AMBIENT298
23D_ 13C-SEPARATED_NOESY1.0MM SH2 U-15N, 13C PHOSPHATE BUFFER NA; 200MM NACL;90%H2O, 10%D2O250mM6.8AMBIENT298
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1VarianINOVA800
NMR Refinement
MethodDetailsSoftware
torsion angle dynamicsCYANA
NMR Ensemble Information
Conformer Selection Criteria
Conformers Calculated Total Number20
Conformers Submitted Total Number1
Representative Model1 (lowest energy)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1refinementCYANA2.0Guetntert P.
2structure solutionXEASY
3structure solutionOlivia
4structure solutionNMRPipe
5structure solutionCYANA2.0Guetntert P.