Experiment: 2EUY

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	2D NOESY	unlabeled	10% D2O, 90% H2O	10mM sodium phosphate buffer	6.0	ambient	293
2	2D NOESY	unlabeled	99.999% D2O	10mM sodium phosphate buffer	6.0	ambient	293
3	2D TOCSY	unlabeled	99.999% D2O	10mM sodium phosphate buffer	6.0	ambient	293
4	3D HCCH-TOCSY	G,A,U,or C-selectively 13C,15N-labled	99.999% D2O	10mM sodium phosphate buffer	6.0	ambient	293
5	3D HCCH-COSY	G,A,U,or C-selectively 13C,15N-labled	99.999% D2O	10mM sodium phosphate buffer	6.0	ambient	293
6	13C-filtered/edited 2D NOESYs	G,A,U,or C-selectively 13C,15N-labled	99.999% D2O	10mM sodium phosphate buffer	6.0	ambient	293

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	DRX	500
2	Bruker	DRX	600

NMR Refinement
Method	Details	Software
distance geometry simulated annealing	The structure was refined against 11 residual dipolar coupling rstraints	XwinNMR

NMR Ensemble Information
Conformer Selection Criteria	structures with the lowest energy
Conformers Calculated Total Number	100
Conformers Submitted Total Number	15
Representative Model	1 (lowest energy)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	collection	XwinNMR	3.5	Bruker
2	data analysis	Felix	2000	Accelry
3	refinement	X-PLOR	NIH	Clore

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.