2ENT

Solution structure of the second C2H2-type zinc finger domain from human Krueppel-like factor 15


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D_13C-separated_NOESY1.18mM uniformly 13C,15N-labeled protein; 20mM TrisHCl; 100mM NaCl; 1mM DTT; 0.02% NaN3; 0.05mM ZnCl2; 1mM IDA; 10% D2O, 90% H2O10% D2O, 90% H2O120mM7.0ambient293
23D_15N-separated_NOESY1.18mM uniformly 13C,15N-labeled protein; 20mM TrisHCl; 100mM NaCl; 1mM DTT; 0.02% NaN3; 0.05mM ZnCl2; 1mM IDA; 10% D2O, 90% H2O10% D2O, 90% H2O120mM7.0ambient293
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1VarianINOVA800
NMR Refinement
MethodDetailsSoftware
torsion angle dynamicsVNMR
NMR Ensemble Information
Conformer Selection Criteriastructures with the least restraint violations, target function
Conformers Calculated Total Number100
Conformers Submitted Total Number20
Representative Model1 (lowest energy)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1collectionVNMR6.1CVarian
2processingNMRPipe20020425Delaglio, F.
3data analysisNMRView5.0.4Johnson, B.A.
4data analysisKUJIRA0.9822Kobayashi, N.
5structure solutionCYANA2.0.17Guntert, P.
6refinementCYANA2.0.17Guntert, P.