2EBT

Solution structure of three tandem repeats of zf-C2H2 domains from human Kruppel-like factor 5

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	3D_13C-separated_NOESY	0.59mM uniformly 13C/15N-labelled protein, 20mM TrisHCl, 100mM NaCl, 1mM DTT, 0.02% NaN3, 50uM ZnCl2, 1mM IDA	10% D2O, 90% H2O	120mM	7.0	ambient	293
2	3D_15N-separated_NOESY	0.59mM uniformly 13C/15N-labelled protein, 20mM TrisHCl, 100mM NaCl, 1mM DTT, 0.02% NaN3, 50uM ZnCl2, 1mM IDA	10% D2O, 90% H2O	120mM	7.0	ambient	293

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Varian	INOVA	900
2	Varian	INOVA	800

NMR Refinement
Method	Details	Software
torsion angle dynamics		VNMR

NMR Ensemble Information
Conformer Selection Criteria	structures with the lowest energy, target function
Conformers Calculated Total Number	100
Conformers Submitted Total Number	20
Representative Model	1 (lowest energy)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	collection	VNMR	6.1C	Varian
2	processing	NMRPipe	20020425	Delaglio, F.
3	data analysis	NMRView	5.0.4	Johnson, B.A.
4	data analysis	KUJIRA	0.9822	Kobayashi, N.
5	structure solution	CYANA	2.0.17	Guntert, P.
6	refinement	CYANA	2.0.17	Guntert, P.

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.