2DO6

Solution structure of RSGI RUH-065, a UBA domain from human cDNA


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D_15N-separated_NOESY1.1mM UBA domain U-15N,13C, 20mM d-Tris-HCl buffer (pH 7.0), 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 90% H2O, 10% D2O90% H2O/10% D2O100mM NaCl7.0AMBIENT298
23D_13C-separated_NOESY1.1mM UBA domain U-15N,13C, 20mM d-Tris-HCl buffer (pH 7.0), 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 90% H2O, 10% D2O90% H2O/10% D2O100mM NaCl7.0AMBIENT298
33D_13C_F1-filtered_F3-edited_NOESY1.0mM UBA domain U-15N,13C, 1.0mM UBA domain, 20mM d-Tris-HCl buffer (pH 7.0), 100mM NaCl, 1mM d-DTT, 0.02% NaN3100% D2O100mM NaCl7.0AMBIENT298
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerAVANCE800
NMR Refinement
MethodDetailsSoftware
torsion angle dynamicsXwinNMR
NMR Ensemble Information
Conformer Selection Criteriatarget function, structures with the lowest energy, structures with the least restraint violations
Conformers Calculated Total Number100
Conformers Submitted Total Number20
Representative Model1 (lowest energy)
Additional NMR Experimental Information
DetailsThis structure was determined using 3D NMR techniques
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1collectionXwinNMR2.6Bruker
2processingNMRPipe20031121Delaglio, F.
3data analysisNMRView5.0.4Johnson, B. A.
4data analysisKUJIRA0.9295Kobayashi, N.
5structure solutionCYANA2.2.2Guntert, P. et al.
6refinementOPALp1.4Koradi, R., Billeter, M., Guntert, P.