2DMI
Solution structure of the first and the second zf-C2H2 like domains of human Teashirt homolog 3
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 3D_15N-separated_NOESY | 1.19mM; 20mM Nad-Mal(6.0),100mM NaCl,0.1mM DTT,0.02%NaN3,0.05mM ZnCl2,1mM NTA | 90% H2O/10% D2O | 120mM | 7.0 | ambient | 293 | |
| 2 | 3D_13C-separated_NOESY | 1.19mM; 20mM Nad-Mal(6.0),100mM NaCl,0.1mM DTT,0.02%NaN3,0.05mM ZnCl2,1mM NTA | 90% H2O/10% D2O | 120mM | 7.0 | ambient | 293 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | INOVA | 800 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| torsion angle dynamics | VNMR | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | target function, structures with the lowest energy, structures with the least restraint violations |
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 20 |
| Representative Model | 1 (lowest energy) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | collection | VNMR | 6.1C | Varian |
| 2 | processing | NMRPipe | 20031121 | Delagilo,F. |
| 3 | data analysis | NMRView | 5.0.4 | Johnson,B.A |
| 4 | data analysis | KUJIRA | 0.955 | Kobayashi,N. |
| 5 | structure solution | CYANA | 2.0.17 | Guntert,P. |
| 6 | refinement | CYANA | 2.0.17 | Guntert,P. |
