2DH9

Solution structure of the C-terminal RNA binding domain in Heterogeneous nuclear ribonucleoprotein M


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D_15N-separated_NOESY20mM d-Tris-HCl(pH 7.0), 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 10% D2O90% H2O/10% D2O100mM7.0ambient298
23D_13C-separated_NOESY20mM d-Tris-HCl(pH 7.0), 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 10% D2O90% H2O/10% D2O100mM7.0ambient298
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerAVANCE800
NMR Refinement
MethodDetailsSoftware
torsion angle dynamics,restrainted molecular dynamicsXwinNMR
NMR Ensemble Information
Conformer Selection Criteriatarget function,structures with the lowest energy,structures with the least restraint violations
Conformers Calculated Total Number100
Conformers Submitted Total Number20
Representative Model1 (lowest energy)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1collectionXwinNMR3.5Bruker
2processingNMRPipe20030801Delaglio,F
3data analysisNMRView5.0.4Johnson,B.A
4data analysisKUJIRA0.932Kobayashi,N
5structure solutionCYANA2.0Guntert,P
6refinementCYANA2.0Guntert,P