2CHK
NMR structure of TLLLLT quadruplex
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | NOESY | D20,10% D20/90% H2O | 100 mM | 7.0 | 1.0 atm | 298.0 | ||
| 2 | COSY | D20,10% D20/90% H2O | 100 mM | 7.0 | 1.0 atm | 298.0 | ||
| 3 | TOCSY | D20,10% D20/90% H2O | 100 mM | 7.0 | 1.0 atm | 298.0 | ||
| 4 | HSQC | D20,10% D20/90% H2O | 100 mM | 7.0 | 1.0 atm | 298.0 | ||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | INOVA | 800 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| simulated annealing | REFINEMENT DETAILS CAN BE FOUND IN JRNL CITATION ABOVE | X-PLOR |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | |
| Conformers Calculated Total Number | 20 |
| Conformers Submitted Total Number | 20 |
| Additional NMR Experimental Information | |
|---|---|
| Details | NONE |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | X-PLOR | 3.1 | BRUNGER |
| 2 | structure solution | X-PLOR | ||
