2BO5
Bovine oligomycin sensitivity conferral protein N-terminal domain
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 2D NOESY | 7% D2O, 93%H2O | 0.5M NACL, 20MM SODIUM PHOSPHATE | 6.5 | 1.0 atm | 300.0 | ||
| 2 | 2D TOCSY | 7% D2O, 93%H2O | 0.5M NACL, 20MM SODIUM PHOSPHATE | 6.5 | 1.0 atm | 300.0 | ||
| 3 | HNCA | 7% D2O, 93%H2O | 0.5M NACL, 20MM SODIUM PHOSPHATE | 6.5 | 1.0 atm | 300.0 | ||
| 4 | CBCACONH | 7% D2O, 93%H2O | 0.5M NACL, 20MM SODIUM PHOSPHATE | 6.5 | 1.0 atm | 300.0 | ||
| 5 | HCCH-COSY | 7% D2O, 93%H2O | 0.5M NACL, 20MM SODIUM PHOSPHATE | 6.5 | 1.0 atm | 300.0 | ||
| 6 | 15N NOESY-HSQC | 7% D2O, 93%H2O | 0.5M NACL, 20MM SODIUM PHOSPHATE | 6.5 | 1.0 atm | 300.0 | ||
| 7 | 13C NOESY-HSQC | 7% D2O, 93%H2O | 0.5M NACL, 20MM SODIUM PHOSPHATE | 6.5 | 1.0 atm | 300.0 | ||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | Avance | 800 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| simulated annealing | REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE. | CNS |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | LOW NOE ENERGY |
| Conformers Calculated Total Number | 50 |
| Conformers Submitted Total Number | 44 |
| Representative Model | 1 (n/a) |
| Additional NMR Experimental Information | |
|---|---|
| Details | THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C, 15N-LABELED OSCP-NT |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | CNS | BRUNGER,ADAMS,CLORE,DELANO,GROS, GROSSE- KUNSTLEVE,JIANG,KUSZEWSKI,NILGES, PANNU,READ, RICE,SIMONSON,WARREN | |
| 2 | structure solution | CNS | ||
