2BAI
The Zinc finger domain of Mengovirus Leader polypeptide
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 3D_13C-separated_NOESY | 1mM NC-labeled mengol, 50mM NaPi, 93% H2O, 7% D2O | 93% H2O/7% D2O | 50mM | 6.5 | ambient | 298 | |
| 2 | 3D_15N-separated_NOESY | 1mM NC-labeled mengol, 50mM NaPi, 93% H2O, 7% D2O | 93% H2O/7% D2O | 50mM | 6.5 | ambient | 298 | |
| 3 | HCCHTOCSY | 1mM NC-labeled mengol, 50mM NaPi, 93% H2O, 7% D2O | 93% H2O/7% D2O | 50mM | 6.5 | ambient | 298 | |
| 4 | HNCA | 1mM NC-labeled mengol, 50mM NaPi, 93% H2O, 7% D2O | 93% H2O/7% D2O | 50mM | 6.5 | ambient | 298 | |
| 5 | HCCONH | 1mM NC-labeled mengol, 50mM NaPi, 93% H2O, 7% D2O | 93% H2O/7% D2O | 50mM | 6.5 | ambient | 298 | |
| 6 | CCONH | 1mM NC-labeled mengol, 50mM NaPi, 93% H2O, 7% D2O | 93% H2O/7% D2O | 50mM | 6.5 | ambient | 298 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | INOVA | 600 |
| 2 | Bruker | DMX | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| torsion angle dynamics | NMRPipe | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the lowest energy |
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 20 |
| Representative Model | 1 (lowest energy) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | processing | NMRPipe | Frank Delaglio | |
| 2 | data analysis | PIPP/STAPP | Dan Garrett | |
| 3 | structure solution | CYANA | 2.0 | Guentert |
| 4 | refinement | CYANA | 2.0 | Guentert |
